Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus

Ye Yuan; Xiuli Wang; Sheping Guo; Yang Liu; Hui Ge; Xuemei Qiu

doi:10.1007/s00343-010-0068-1

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Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus

OriginalPaper | Updated：2023-05-16

- Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus
- Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus
- 海洋湖沼学报（英文） 2010年28卷第6期页码：1254-1260
- Affiliations：
  
  College of Life Sciences and Biotechnology Dalian Fisheries University
- Author bio：
- Funds：
  
  Supported by the Dalian Municipal Government of China (No. 2007B11NC069), the Key Laboratory Foundation of the Educational Department of Liaoning Province (No.2009S024), and the Grant of Dalian Fisheries University (No. SY2007005)
- DOI：10.1007/s00343-010-0068-1
  中图分类号：
- 网络首发：2010-06-24，
  
  纸质出版：2010
- Accepted：
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Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus[J]. 海洋湖沼学报（英文）, 2010,28(6):1254-1260.

Yuan, Y., Wang, X., Guo, S. et al. Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus, ., Chin. J. Ocean. Limnol. 28, 1254–1260 (2010). https://doi.org/10.1007/s00343-010-0068-1
Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus[J]. 海洋湖沼学报（英文）, 2010,28(6):1254-1260. DOI： 10.1007/s00343-010-0068-1.

Yuan, Y., Wang, X., Guo, S. et al. Gene cloning and prokaryotic expression of recombinant flagellin A from Vibrio parahaemolyticus, ., Chin. J. Ocean. Limnol. 28, 1254–1260 (2010). https://doi.org/10.1007/s00343-010-0068-1 DOI：

摘要

Abstract

The Gram-negative Vibrio parahaemolyticus is a common pathogen in humans and marine animals. Bacteria flagellins play an important role during infection and induction of the host immune response. Thus

flagellin proteins are an ideal target for vaccines. We amplified the complete flagellin subunit gene (flaA) from V. parahaemolyticus ATCC 17802. We then cloned and expressed the gene into Escherichia coli BL21 (DE3) cells. The gene coded for a protein that was 62.78 kDa. We purified and characterized the protein using Ni-NTA affinity chromatography and Anti-His antibody Western blotting

respectively. Our results provide a basis for further studies into the utility of the FlaA protein as a vaccine candidate against infection by Vibrio parahaemolyticus. In addition

the purified FlaA protein can be used for further functional and structural studies.

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references

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