Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye

Jinhua Feng; Huahua Yu; Cuiping Li; Ronge Xing; Song Liu; Lin Wang; Shengbao Cai; Pengcheng Li

doi:10.1007/s00343-009-9225-9

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Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye

OriginalPaper | Updated：2023-05-16

- Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye
- Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye
- 海洋湖沼学报（英文） 2009年27卷第4期页码：869-874
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by Qingdao Science and Technology Project (No. 08-1-3-51-JCH) and the Award Foundation of Scientific Research for Excellent Young and Middle-Age Scientist of Shandong Province (No. 2006BS 07003)
- DOI：10.1007/s00343-009-9225-9
  中图分类号：
- 网络首发：2009-12-04，
  
  纸质出版：2009
- Accepted：
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Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye[J]. 海洋湖沼学报（英文）, 2009,27(4):869-874.

Feng, J., Yu, H., Li, C. et al. Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye., Chin. J. Ocean. Limnol. 27, 869–874 (2009). https://doi.org/10.1007/s00343-009-9225-9
Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye[J]. 海洋湖沼学报（英文）, 2009,27(4):869-874. DOI： 10.1007/s00343-009-9225-9.

Feng, J., Yu, H., Li, C. et al. Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye., Chin. J. Ocean. Limnol. 27, 869–874 (2009). https://doi.org/10.1007/s00343-009-9225-9 DOI：

摘要

Abstract

The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified: mastigophores

euryteles

and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa

and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L

pH 7.8

5% glycerol

0.5 mmol/L EDTA

0.1 mol/L NaCl). The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 µg/mL.

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references

Berking S. Herrmann K. 2006. Formation and discharge of nematocysts is controlled by protein gradient across the cyst membrane. Helgol. Mar. Res., 60: 180–188.

Blanquet R S. 1970. Ionic effects on discharge of the isolated and in situ nematocysts of the sea anemone Aiptasia pallida: a possible role of calcium. Comp. Biochem. Physiol., 35: 451–461.

Bloom D A, Burnett J W, Alderslade P. 1998. Partial purification of box jellyfish (Chironex fleckeri) nematocyst venom isolated at the beachside. Toxicon, 36: 1075–1085.

Bradford J W. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254.

Burnett J W, Ordonez J V, Calton G J. 1986. Differential toxicity of physalia physalis (Portuguese man-o’war) nematocysts separated by flow cytometry. Toxicon, 24: 514–518.

Hessinger D A, Lenhoff H M. 1988. The biology of nematocysts, Academic Press, San Diego, CA.

Hong H X. 2002. Jellyfish. Bulletin of Biology, 37(2): 13–16.

Laemmli U K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227: 680–685.

Li C P, Yu H H, Liu S, Xing R E, Guo Z Y, Li P C. 2005. Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye. Bioorg. Med. Chem. Lett., 15: 5370–5374.

Maridcal R N. 1974. Nematocysts. In: Lenhoff, H., L. Muscatine, Coelenterate Biology: Reviews and Perspectives. Academic, San Diego, p. 129–178.

Nagai H, Takuwa K, Nakao M, Ito M, Miyake M, Noda M, Nakajima T. 2000a. Novel proteinaceous toxins from the box jellyfish (Sea wasp) Carybdea rastoni. Biochem. Biophys. Res. Commun., 275: 582–588.

Nagai H, Takuwa K, Nakao M, Oshiro N, Iwanaga S, Nakajima T. 2000b. A novel protein toxin from the deadly box jellyfish (sea wasp, Habu-kurage) Chiropsalmus quadrigatus. Biosci. Biotechnol. Biochem., 66(1): 97–102.

Phillips J H. 1956. Isolation of active nematocysts of Metridium senile and their chemical composition. Nature, 178: 932.

Radwan F F Y, Burnett J W. 2001. Toxinological studies of the venom from Cassiopea xamachana nematocysts isolated by flow cytometry. Comp. Biochem. Physiol. C, 128: 65–73.

Ramasamy S, Isbister G K, Seymour J E, Hodgson W C. 2005. Pharmacologically distinct cardiovascular effects of box jellyfish (Chironex fleckeri) venom and a tentacle-only extract in rats. Toxicol. Lett., 155: 219–226.

Rottini G, Gusmani L, Parovel E, Avian M, Patriarca P. 1995. Purification and properties of a cytolytic toxin in venom of the jellyfish Carubdea marsupialis. Toxicon, 33: 315–326.

Spackman D H, Stein W H, Moore S. 1958. Automatic recording apparatus for use in the chromatography of amino acids. Anal. Chem., 30: 1190–1206.

Toom P M, Chan D S. 1972. Preliminary studies of nematocysts from the jellyfish Stomolophus meleagris. Toxicon., 10: 605–610.

Yu H H, Liu X G, Xing R E, Liu S, Guo Z Y, Wang P B, Li C P, Li P C. 2006. In vitro determination of antioxidant activity of proteins from jellyfish Rhopilema esculentum. Food Chem., 95: 123–130.

Yu H H, Li C P, R G Li, Liu S, Li P C. 2007. Factors affecting hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye. Food Chem. Toxicol., 45(7): 1173–1178.

Yu H H, Liu X G, Dong X L, Li C P, Xing, Liu S, Li P C. 2005. Insecticidal activity of proteinous venom from tentacle of jellyfish Rhopilema esculentum Kishinouye. Bioorg. Med. Chem. Lett., 15: 4949–4952.

Weber J. 1990. Poly (γ-glutamic Acid) are the major constituents of nematocysts in Hydra (Hydrozoa, Cnidaria). J. Biol. Chem., 265: 9664–9669.

Wittle L W. 1974. Stinging coral (Millepora tenera) toxin: a comparison of crude extracts with isolated nematocyst extracts. Toxicon., 12: 481–486.

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