The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production

Jing Li; Zhenming Chi; Xianghong Wang; Ying Peng; Zhe Chi

doi:10.1007/s00343-009-9198-8

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The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production

OriginalPaper | Updated：2023-05-16

- The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production
- The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production
- 海洋湖沼学报（英文） 2009年27卷第4期页码：753-761
- Affiliations：
  
  UNESCO Chinese Center of Marine Biotechnology Ocean University of China
- Author bio：
- Funds：
  
  Supported by the High Technology Research and Development Program of China (863 Program) (No. 2006AA09Z403) and the National Science and Technology Infrastructure Program of China (No. 2005DKA21209)
- DOI：10.1007/s00343-009-9198-8
  中图分类号：
- 网络首发：2009-12-04，
  
  纸质出版：2009
- Accepted：
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The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production[J]. 海洋湖沼学报（英文）, 2009,27(4):753-761.

Li, J., Chi, Z., Wang, X. et al. The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production., Chin. J. Ocean. Limnol. 27, 753–761 (2009). https://doi.org/10.1007/s00343-009-9198-8
The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production[J]. 海洋湖沼学报（英文）, 2009,27(4):753-761. DOI： 10.1007/s00343-009-9198-8.

Li, J., Chi, Z., Wang, X. et al. The selection of alkaline protease-producing yeasts from marine environments and evaluation of their bioactive peptide production., Chin. J. Ocean. Limnol. 27, 753–761 (2009). https://doi.org/10.1007/s00343-009-9198-8 DOI：

摘要

Abstract

A total of 400 yeast strains from seawater

sediments

saltern mud

marine fish guts

and marine algae were obtained. The protease activity of the yeast cultures was estimated

after which four strains (HN3.11

N11b

YF04C and HN4.9) capable of secreting extracellular alkaline protease were isolated. The isolated strains were identified as Aureobasidium pullulans

Yarrowia lipolytica

Issatchenkia orientalis and Cryptococcus cf. aureus. The optimal pH of the protease activity produced by strains HN3.11

YF04C

and HN4.9 was 9.0

while that of the protease produced by strain N11b was 10.0. The optimal temperature for protease activity was 45°C for strains HN3.11

N11b

and YF04C

and 50°C for strain HN4.9. After digestion of shrimp (Penaeus vannamei) protein and spirulina (Arthospira platensis) protein with the four crude alkaline proteases

the filtrate from spirulina (Arthrospira platensis) powder digested by the crude alkaline protease of strain HN3.11 was found to have the highest antioxidant activity (61.4%) and the highest angiotensin I converting enzyme (ACE)-inhibitory activities (68.4%). The other filtrates had much lower antioxidant activity and ACE-inhibitory activities.

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references

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