Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae

Wenbin Zhan; Hongming Liu; Jing Xing; Xiuzhen Sheng; Xiaoqian Tang

doi:10.1007/s00343-009-9195-y

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Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae

OriginalPaper | Updated：2023-05-16

- Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae
- Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae
- 海洋湖沼学报（英文） 2009年27卷第3期页码：536-542
- Affiliations：
  
  Laboratory of Pathology and Immunology of Aquatic Animals LMMEC Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program, No. 2006CB101806), the High Technology Research and Development Program of China (863 Program, No. 2006AA100306) and the National Natural Science Fundation of China (No. 30771648)
- DOI：10.1007/s00343-009-9195-y
  中图分类号：
- 网络首发：2009-11-07，
  
  纸质出版：2009
- Accepted：
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Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae[J]. 海洋湖沼学报（英文）, 2009,27(3):536-542.

Zhan, W., Liu, H., Xing, J. et al. Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae, ., Chin. J. Ocean. Limnol. 27, 536–542 (2009). https://doi.org/10.1007/s00343-009-9195-y
Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae[J]. 海洋湖沼学报（英文）, 2009,27(3):536-542. DOI： 10.1007/s00343-009-9195-y.

Zhan, W., Liu, H., Xing, J. et al. Variation in the immunoglobulin levels in turbot (Scophthalmus maximus) after vaccination with Streptococcus iniae, ., Chin. J. Ocean. Limnol. 27, 536–542 (2009). https://doi.org/10.1007/s00343-009-9195-y DOI：

摘要

Abstract

A pathogenic bacterium (S636)

identified as Streptococcus iniae

was isolated from turbot (Scophthalmus maximus) in 2005. We immunized turbot with formalin-killed S. iniae four times (on days 1

and 28) by intraperitoneal inoculation. After each vaccination

we obtained serum samples and isolated the lymphocytes from the peripheral blood

spleen

pronephros

and mesonephros. We measured surface Ig-positive (sIg+) lymphocytes and serum antibody levels from these organs using flow cytometry and enzyme-linked immunosorbent assay (ELISA)

respectively

using monoclonal antibodies against turbot immunoglobulin. We confirmed that the antibody reacted with both the surface and plasma Ig by confocal laser scanning microscopy and electron microscopy. The percentage of sIg+ in the lymphocytes increased following each successive vaccination. The mean percentage increased from 31.96% (control) to 37.49%

38.36%

42.9%

and 51.63% in the peripheral blood; from 27.09% to 36.63%

36.81%

39.28%

and 46.0% in the spleen; from 22.2% to 28.99%

29.21%

32.83%

and 41.58% in pronephros; and from 18.12% to 22.17%

22.45%

25.69%

and 31.68% in the mesonephros. The ELISA results were consistent with these results. Both the total and specific antibody levels increased with each vaccination. The mean OD value of the specific antibody assay increased from 0.094

to 0.269

0.283

0.333

and 0.421; for total antibody the mean OD value increased from 0.133

to 0.292

0.323

0.413

and 0.527.

关键词

Keywords

references

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