The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity

Yongchao Guo; Zhenxing Li; Hong Lin

doi:10.1007/s00343-009-9190-3

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The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity

OriginalPaper | Updated：2023-05-16

- The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity
- The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity
- 海洋湖沼学报（英文） 2009年27卷第4期页码：703-707
- Affiliations：
  
  Food Safety Laboratory Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (NO.2006AA09Z427) and the National Natural Science Foundation of China (Nos. 30800859, 30871948)
- DOI：10.1007/s00343-009-9190-3
  中图分类号：
- 网络首发：2009-12-04，
  
  纸质出版：2009
- Accepted：
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The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity[J]. 海洋湖沼学报（英文）, 2009,27(4):703-707.

Guo, Y., Li, Z. & Lin, H. The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity., Chin. J. Ocean. Limnol. 27, 703–707 (2009). https://doi.org/10.1007/s00343-009-9190-3
The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity[J]. 海洋湖沼学报（英文）, 2009,27(4):703-707. DOI： 10.1007/s00343-009-9190-3.

Guo, Y., Li, Z. & Lin, H. The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity., Chin. J. Ocean. Limnol. 27, 703–707 (2009). https://doi.org/10.1007/s00343-009-9190-3 DOI：

摘要

Abstract

Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp (Penaeus vannamei) was subjected to digestion in SGF (simulated gastric fluid) and SIF (simulated intestinal fluid) for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA

using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However

Pen a l exhibited strong resistance to digestive fluids

and all yielded fragments (33 kD

23 kD

and 14 kD) showed allergic activity. Therefore

anti-digestion may be an important factor for Pen a 1 to induce an allergy.

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Keywords

references

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