Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system

Dan Yi; Xiuqin Sun; Guangyou Li; Fayi Liu; Xuezheng Lin; Jihong Shen

doi:10.1007/s00343-009-9170-7

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Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system

OriginalPaper | Updated：2023-05-16

- Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system
- Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system
- 海洋湖沼学报（英文） 2009年27卷第3期页码：574-577
- Affiliations：
  
  1. College of Life Sciences Ocean University of China
  2. Key Laboratory of Marine Bio-active Substance First Institute of Oceanography State Oceanic Administration
- Author bio：
- Funds：
  
  Supported by the High Technology Research and Development Program of China (No. 2007AA091905) and the Technical Program of Municipal Technology Bureau Qingdao (No. 04-2-JS-117)
- DOI：10.1007/s00343-009-9170-7
  中图分类号：
- 网络首发：2009-11-07，
  
  纸质出版：2009
- Accepted：
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Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system[J]. 海洋湖沼学报（英文）, 2009,27(3):574-577.

Yi, D., Sun, X., Li, G. et al. Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system., Chin. J. Ocean. Limnol. 27, 574–577 (2009). https://doi.org/10.1007/s00343-009-9170-7
Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system[J]. 海洋湖沼学报（英文）, 2009,27(3):574-577. DOI： 10.1007/s00343-009-9170-7.

Yi, D., Sun, X., Li, G. et al. Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system., Chin. J. Ocean. Limnol. 27, 574–577 (2009). https://doi.org/10.1007/s00343-009-9170-7 DOI：

摘要

Abstract

We compared four esterifiable enzymes. The lipase Novozym 435 possessed the highest activity for the conjugated linoleic acid esterification during the synthesis of triglycerides. The triglycerides were synthesized by esterification of glycerol and conjugated linoleic acid (CLA) in a no-solvent system using lipase catalysis. We investigated the effects of temperature

enzyme concentration

water content

and time on esterification. Enzyme and water concentrations of up to 1% of the total reaction volume and a system temperature of 60°C proved optimal for esterification. Similarly

when the esterification was carried out for 24 h

the reaction ratio improved to 94.11%. The esterification rate of the rotating screen basket remained high (87.28%) when the enzyme was re-used for the 5th time. We evaluated the substrate selectivity of lipase (NOVO 435) and determined that this lipase prefers the 10

12-octadacadienoic acid to the 9

11-octadecadienoic acid.

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references

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