Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis

Yanyan Li; Songdong Shen; Lihong He; Pu Xu; Guangce Wang

doi:10.1007/s00343-009-9168-1

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Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis

OriginalPaper | Updated：2023-05-16

- Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis
- Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis
- 海洋湖沼学报（英文） 2009年27卷第3期页码：493-501
- Affiliations：
  
  1. Department of Cell Biology Medical College of Soochow University
  2. Department of Biology and Food Engineering Changshu Institute of Technology
  3. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 40576074), and the Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences (No. KFN92007NO1)
- DOI：10.1007/s00343-009-9168-1
  中图分类号：
- 网络首发：2009-11-07，
  
  纸质出版：2009
- Accepted：
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Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis[J]. 海洋湖沼学报（英文）, 2009,27(3):493-501.

Li, Y., Shen, S., He, L. et al. Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis, ., Chin. J. Ocean. Limnol. 27, 493–501 (2009). https://doi.org/10.1007/s00343-009-9168-1
Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis[J]. 海洋湖沼学报（英文）, 2009,27(3):493-501. DOI： 10.1007/s00343-009-9168-1.

Li, Y., Shen, S., He, L. et al. Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis, ., Chin. J. Ocean. Limnol. 27, 493–501 (2009). https://doi.org/10.1007/s00343-009-9168-1 DOI：

摘要

Abstract

The sequences of the ITS (internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli (NB

PT and ST) were amplified

sequenced and analyzed. In addition

the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated. The results are as follows: the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains. The sequences of ITS1 were 331 bp to 334 bp

while those of the 5.8S rDNA were 158 bp and the sequences of ITS2 ranged from 673 bp to 681 bp. The sequences of the ITS had a high level of homology (up to 99.5%) with that of P. haitanensis (DQ662228) retrieved from GenBank

but were only approximately 50% homologous with those of other species of Porphyra. The results obtained when a phylogenetic tree was constructed coincided with the results of the homology analysis. These results suggest that the three cultivated strains of P. haitanensis evolved conservatively and that the ITS showed evolutionary consistency. However

the sequences of the ITS and 5.8S rDNA of different Porphyra species showed great variations. Therefore

the relationship of Porphyra interspecies phyletic evolution could be judged

which provides the proof for Porphyra identification study. However

proper classifications of the subspecies and the populations of Porphyra should be determined through the use of other molecular techniques to determine the genetic variability and rational phylogenetic relationships.

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references

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