Isolation and identification of bacteria associated with the surfaces of several algal species

Zifeng Wang; Tian Xiao; Shaojun Pang; Min Liu; Haidong Yue

doi:10.1007/s00343-009-9165-4

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Isolation and identification of bacteria associated with the surfaces of several algal species

OriginalPaper | Updated：2023-05-16

- Isolation and identification of bacteria associated with the surfaces of several algal species
- Isolation and identification of bacteria associated with the surfaces of several algal species
- 海洋湖沼学报（英文） 2009年27卷第3期页码：487-492
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School of the Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 40376048) and the National Basic Research Program of China (973 Program) (No. 2006CB400604)
- DOI：10.1007/s00343-009-9165-4
  中图分类号：
- 网络首发：2009-11-07，
  
  纸质出版：2009
- Accepted：
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Isolation and identification of bacteria associated with the surfaces of several algal species[J]. 海洋湖沼学报（英文）, 2009,27(3):487-492.

Wang, Z., Xiao, T., Pang, S. et al. Isolation and identification of bacteria associated with the surfaces of several algal species., Chin. J. Ocean. Limnol. 27, 487–492 (2009). https://doi.org/10.1007/s00343-009-9165-4
Isolation and identification of bacteria associated with the surfaces of several algal species[J]. 海洋湖沼学报（英文）, 2009,27(3):487-492. DOI： 10.1007/s00343-009-9165-4.

Wang, Z., Xiao, T., Pang, S. et al. Isolation and identification of bacteria associated with the surfaces of several algal species., Chin. J. Ocean. Limnol. 27, 487–492 (2009). https://doi.org/10.1007/s00343-009-9165-4 DOI：

摘要

Abstract

We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses. Twelve strains of bacteria were obtained from the surfaces of the following four species of algae: Gracilaria textorii

Ulva pertusa

Laminaria japonica

and Polysiphonia urceolata. The isolated strains of bacteria can be divided into two groups: Halomonas and Vibrio

in physiology

biochemical characteristics and 16S rDNA sequence analyses. The phylogenetic tree constructed based on 16S rDNA sequences of the isolates shows four obvious clusters

Halomonas venusta

Vibrio tasmaniensis

Vibrio lentus

and Vibrio splendidus. Isolates from the surface of P. urceolata are more abundant and diverse

of which strains P9 and P28 have a 16S rDNA sequence very similar (97.5%–99.8%) to that of V. splendidus. On the contrary

the isolates from the surfaces of G. textorii

U. pertusa and L. japonica are quite simple and distribute on different branches of the phylogenetic tree. In overall

the results of this study indicate that the genetic relationships among the isolates are quite close and display a certain level of host species specificity

and alga-associated bacteria species are algal species specific.

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references

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