Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides

Jianfeng Niu; Guangce Wang; Fang Lü; Baicheng Zhou; Guang Peng

doi:10.1007/s00343-009-9157-4

Your Location：

Home >

Browse articles >

Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides

OriginalPaper | Updated：2023-05-16

- Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
- Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
- 海洋湖沼学报（英文） 2009年27卷第3期页码：502-512
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. College of Marine Science and Engineering Tianjin University of Science and Technology
  3. The Graduate School of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of China (Nos. 40806063, 30830015) and the National High Technology Research and Development Program of China (863 Program) (Nos.2007AA09Z406, 2006AA10A413)
- DOI：10.1007/s00343-009-9157-4
  中图分类号：
- 网络首发：2009-11-07，
  
  纸质出版：2009
- Accepted：
Scan QR Code
Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides[J]. 海洋湖沼学报（英文）, 2009,27(3):502-512.

Niu, J., Wang, G., Lü, F. et al. Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides, ., Chin. J. Ocean. Limnol. 27, 502–512 (2009). https://doi.org/10.1007/s00343-009-9157-4
Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides[J]. 海洋湖沼学报（英文）, 2009,27(3):502-512. DOI： 10.1007/s00343-009-9157-4.

Niu, J., Wang, G., Lü, F. et al. Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides, ., Chin. J. Ocean. Limnol. 27, 502–512 (2009). https://doi.org/10.1007/s00343-009-9157-4 DOI：

摘要

Abstract

A group of coenocytic marine algae differs from higher plants

whose totipotency depends on an intact cell (or protoplast). Instead

this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals. It is thought that lectins play a key role in the aggregation process. We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides. The lectin was ca. 27 kDa with a pI between pH 5 and pH 6. The absence of carbohydrate suggested that the lectin was not a glycoprotein. The hemagglutinating activity (HA) of the lectin was not dependent on the presence of divalent cations and was inhibited by N-Acetylgalactosamine

N-Acetylglucosamine

and the glycoprotein bovine submaxillary mucin. The lectin preferentially agglutinated Gram-negative bacterium. The HA of this lectin was stable between pH 4 to pH 10. Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution (0.5 mg ml−1). Our results suggest that the regeneration of B. hypnoides is mediated by this lectin. We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater (pH 8.0). Given that nigericin dissipates proton gradients across the membrane

we hypothesize that the aggregation of cell organelles was proton-gradient dependent.

关键词

Keywords

references

Belogortseva N I, Molchanova V I, Kurika A V, Skobun A S, Glazkova V E. 1998. Isolation and characterization of new GalNAc/Gal specific lectin from the sea mussel Crenomytilus grayanus. Comp. Biochem. Physiol., 119: 45–50.

Benevides N M B, Holanda M L, Melo F R, Freitas A L P, Sampaio A H. 1998. Purification and partial characterisation of the lectin from the marine red alga Enantiocladia duperreyi (C. agardh) Falkenberg. Botanica Marina., 41: 521–525.

Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254.

Cominetti M R, Marques M R F, Lorenzini D M, Löfgren S E, Daffre S, Barracco M A. 2002. Characterization and partial purification of a lectin from the hemolymph of the white shrimp Litopenaeus schmitti. Dev. Comp. Immunol., 26: 715–721.

Dai C J, Wang G Z, He J F, Li S J, Huang H Y. 2006. Purification and characterization of lectin from humoral fluids of Charybdis feriatus. Chinese Journal of Oceanology and Limnology, 24: 390–394.

Dubois M, Gilles K A, Hamilton J K, Rebers P A, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Biochem., 28: 350–356.

Halbig D, Hou B, Freudl R, Sprenger G A, Klõsgen R B. 1999. Bacterial protein carrying twin-R signal peptides are specifically targeted by the ΔpH-dependent transport machinery of the thylakoid membrane system. FEBS Lett., 447: 95–98.

Herrler G, Reuter G, Rott R, Klenk H D, Schauer R. 1987. N-acetyl-9-O-acetylneuraminic acid, the receptor determinant for influenza C virus, is a differentiation marker on chicken erythrocytes. Biol. Chem. Hoppe. Seyler., 368: 451–454.

Hori K, Miyazawa K, Ito K. 1987. A mitogenic agglutinin from the red alga Carpopeltis flabelata. Phytochemistry, 26: 1 335–1 338.

Hori K, Miyazawa K, Ito K. 1990. Some common properties of lectins from marine algae. Hydrobiologia, 204/205: 561–566.

Joshi J P. 1987. Putative polyadenylation signals in nuclear genes of higher plants: a comparison and analysis. Nucleic Acids Res., 15: 9 627–9 640.

Kim G H, Klotchkova T A, Kang Y M. 2001. Life without a cell membrane: regeneration of protoplasts from disintegrated cells of the marine green alga Bryopsis plumose. J. Cell Sci., 114: 2 009–2 014.

Kim G H, Klotchkova T A, West J A. 2002. From protoplasm to swarmer: regeneration of protoplasts from disintegrated cells of the multicellular marine green alga Microdictyon umbilicatum (Chlorophyta). J. Phycol., 38: 174–178.

Klotchkova T A, Kim G H. 2006. Purification and characterization of a lectin, Bryohealin, involved in the protoplast formation of a marine green alga Bryopsis plumose. J. Phycol., 42: 86–95.

Klotchkova T A, Chah O K, West J A, Kim G H. 2003. Cytochemical and ultrastructural studies on protoplast formation from disintegrated cells of a marine green alga Chaetomorpha aerea (Chlorophyta). Eur. J. Phycol., 38: 205–216.

Kobayashi K, Kanaizuka Y. 1985. Reunification of sub-cellular fractions of Bryopsis into viable cells. Plant Sci., 40: 129–135.

Laemmli U K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680–685.

Mariani-Colombo P, Vannini G L, Mares D. 1980. A cytochemical approach to the wound repair mechanism in Udotea petiolata (Siphonales). Protoplasma, 104: 105–117.

O’Neil R M, La Claine J W. 1984. Mechanical wounding induces the formation of extensive coated membranes in giant cell. Science, 255: 331–333.

Pak J Y, Solorzano C, Arai M, Nitta T. 1991. Two distinct steps for spontaneous generation of subprotoplasts from a disintegrated Bryopsis cell. Plant Physiol., 96: 819–825.

Peltier J B, Friso G, Kalume D E, Roepstorff P, Nilsson F, Adamska I, Wijk K J. 2000. Proteomics of the chloroplast: systematic identification and targeting analysis of lumenal and peripheral thylakoid proteins. Plant Cell, 12: 319–342.

Peumans W J, W J N VAN Damme. 1995. Lectin as plant defense proteins. Plant Physiol., 109: 347–352.

Rice K G, 1997. Glycoconjugate-medicated drug targeting. In:H. J. Gabius and S. Gabius, eds. Glycosciences: Status and Perspectives. Weinheim: Chapman & Hall, London. p. 471–483.

Rogers D J, Hori K. 1993. Marine algal lectins: new developments. Hydrobiologia, 260/261: 589–593.

Sabine M, Ivan K, Constanze W, Herrmann R G, Klõsgen R B. 2001. The rieske Fe/S protein of the cytochrome b6/f complex in chloroplasts. J. Biol. Chem., 46: 42 761–42 766.

Schauer R, Casalsst J, Corfield A P, Veh R W. 1988. Subcellular site of the biosynthesis of O-acetylated sialic acids in bovine submandibular gland. Glycocon. J., 5: 257–270.

Sharon N, Lis H. 1989. Lectins as cell recognition molecules. Science, 177: 949–959.

Shinji H, Masahide K, Kentaro M, Yoshio T, Shigehisa H. 2000. Multiplicity, structures, and endocrine and exocrine natures of eel fucose-binding lectins. J. Biol. Chem., 275: 33 151–33 157.

Shiomi K, Yamanaka H, Kikuchi T. 1981. Purification and physicochemical properties of a hemagglutinin (GVA-1) in the red alga Gracilaria verrucosa. Bulletin of the Japanese Society of Scientific Fisheries, 47: 1 079–1 084.

Sun J, Wang L, Wang B J, Guo Z Y, Liu M, Jiang K Y, Luo Z Y. 2007. Purification and characterisation of a natural Lectin from the serum of the shrimp Litopenaeus vannamei. Fish Shellfish Immun., 23: 292–299.

Tatewaki M, Nagata K. 1970. Surviving protoplasts in vitro and their development in Bryopsis. J. Phycol., 6: 401–403.

Ueda R, Sugeta H, Degudei Y. 1991. Naturally occurring agglutinin in the hemolymph of Japanese coastal crustacean. Nippon Suisan Gakk., 57: 69–78.

Walker M B, Roy L M, Coleman E, Voelker R, Barkan A. 1999. The maize tha4 gene functions in sec-independent protein transport in chloroplasts and is related to hcf106, tatA, and tatB. J. Cell. Biol., 147: 267–276.

Wang G C, Tseng C K. 2006. Culturing the segments of Bryopsis hypnoides Lamouroux thalli regenerated from protoplasts aggregation. J. Integrat. Plant. Biol., 48: 190–196.

Yao C L, Wu C G, Xiang J H, Dong B. 2005. Molecular cloning and response to laminarin stimulation of arginine kinase in haemolymph in Chinese shrimp, Fennero-penaeus chinensis. Fish Shellfish Immun., 19: 317–329.

Ye N H, Wang G C, Wang F Z, Zeng C K. 2005. Formation and growth of Bryopsis hypnoides Lamouroux Regenerated from its protoplasts. J. Integrat. Plant. Biol., 47: 856–862.

Yoon K S, Lee K P, Klotchkova T A, Kim G H. 2008. Molecular characterization of the lectin, Bryohealin, involved in protoplast regeneration of the marine alga Bryopsis plumosa (Chlorophyta). J. Phycol., 44: 103–112.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Taxonomy and regeneration of a newly recorded Polychaete Capitella teleta (Annelida, Capitellidae) in the coastal water of Shandong, China

High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)

Explant exenisation for tissue culture in marine macroalgae

Dynamic studies on flagellar regeneration inDunaliella salina

Influence of albumen on aggregation and O2 evolution of protoplasm from Bryopsis hypnoides Lamourou