Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus

Jiye Li; Xiuqin Sun; Fengrong Zheng; Hushan Sun

doi:10.1007/s00343-009-9125-z

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Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus

OriginalPaper | Updated：2023-05-16

- Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus
- Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus
- 海洋湖沼学报（英文） 2009年27卷第3期页码：550-554
- Affiliations：
  
  1. State Oceanic Administration’s North China Sea Environmental Monitoring Center
  2. Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology SOA
  3. The First Institute of Oceanography SOA
  4. College of Life Science Ludong University
- Author bio：
- Funds：
  
  Supported by the Special Financed Project from the Basic Public Scientific Research Expenses of Central Scientific Institutes (No. GY02-2007B03)
- DOI：10.1007/s00343-009-9125-z
  中图分类号：
- 网络首发：2009-11-07，
  
  纸质出版：2009
- Accepted：
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Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus[J]. 海洋湖沼学报（英文）, 2009,27(3):550-554.

Li, J., Sun, X., Zheng, F. et al. Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 27, 550–554 (2009). https://doi.org/10.1007/s00343-009-9125-z
Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus[J]. 海洋湖沼学报（英文）, 2009,27(3):550-554. DOI： 10.1007/s00343-009-9125-z.

Li, J., Sun, X., Zheng, F. et al. Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 27, 550–554 (2009). https://doi.org/10.1007/s00343-009-9125-z DOI：

摘要

Abstract

We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles

papillate podium

integument

respiratory tree

and digestive tract and stained them for acid phosphatase (ACP)

alkaline phosphatase (AKP)

non-specific esterase (NSE) and peroxidase (POD) activity. The distribution and content of ACP

AKP

NSE

and POD differed among the tissues. The coelomic epithelium of the tentacle

papillate podium

and integument and the mucous layer of respiratory tree were positive for ACP activity. The coelomic epithelium and cuticular layer of the tentacle

papillate podium

and integument and the mucous layer and tunica externa of the respiratory tree and digestive tract stained positive or weakly positive for AKP activity. Almost all the epithelial tissues stained positive

strongly positive

or very strongly positive for NSE activity. The cuticular layer of the tentacle and integument and the mucous layer

tunica submucosa

and tunica externa of the respiratory tree and digestive tract stained positive for POD activity. We hypothesize that these enzymes play a role in the immune response in A. japonicus.

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references

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Liu Z H, Mou H J, Wang Q Y. 2003. Research progress of immune related enzymes in mollusca. Marine Fisheries Research, 24(3): 86–90

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Qiao J H, CHENG B. 2005. The situation and expection of artificial pond culture for Apostichopus joponicus (Selenlca). Marine Sciences, 29(9): 80–82.

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Wang Y Y, Sun S H, Sun X Q, Li G Y. 2004. Peroxidase activity in gill and labial palps of scallop Chlamys farreri. Journal of Fishery Sciences of China, 11(6): 513–518.

Zhang C Y, Wang Y G, Rong X J. 2006. Isolation and identification of causative pathogen for skin ulcerative syndrome in Apostichopus japonicus. Journal of Fishery Sciences of China, 30(1): 118–123. (in Chinese with English abst ract)

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