Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri

Gaowa Saren; Yonggang Zhao

doi:10.1007/s00343-009-9120-4

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Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri

OriginalPaper | Updated：2023-05-16

- Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri
- Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri
- 海洋湖沼学报（英文） 2009年27卷第2期页码：324-330
- Affiliations：
  
  1. Department of Marine Biology Ocean University of China
  2. Key Laboratory of Marine Geology and Environment Institute of Oceanology Chinese Academy of Sciences
  3. China Animal Health and Epidemiology Center
- Author bio：
- Funds：
  
  Supported by the Pilot Projects of Knowledge Innovation Project of Chinese Academy of Sciences (No. KZCX2-YW-211-03 and MGE2008KG06)
- DOI：10.1007/s00343-009-9120-4
  中图分类号：
- 网络首发：2009-07-12，
  
  纸质出版：2009
- Accepted：
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Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri[J]. 海洋湖沼学报（英文）, 2009,27(2):324-330.

Saren, G., Zhao, Y. Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri, ., Chin. J. Ocean. Limnol. 27, 324–330 (2009). https://doi.org/10.1007/s00343-009-9120-4
Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri[J]. 海洋湖沼学报（英文）, 2009,27(2):324-330. DOI： 10.1007/s00343-009-9120-4.

Saren, G., Zhao, Y. Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri, ., Chin. J. Ocean. Limnol. 27, 324–330 (2009). https://doi.org/10.1007/s00343-009-9120-4 DOI：

摘要

Abstract

A full length amphioxus cDNA

encoding a novel phosducin-like protein (Amphi-PhLP)

was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids

with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary

and at a lower level in the hind gut and testis

hepatic caecum

gill

endostyle

and epipharyngeal groove

while it was absent in the muscle

neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-N1/Amphi-PhLP

the fusion protein was targeted in the cytoplasm of CHO cells

suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.

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references

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