Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus

Yanjie Chen; Quanqi Zhang; Jie Qi; Yeying Sun; Qiwang Zhong; Xubo Wang; Zhigang Wang; Shuo Li; Chunmei Li

doi:10.1007/s00343-009-0092-1

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Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus

OriginalPaper | Updated：2023-05-16

- Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus
- Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus
- 海洋湖沼学报（英文） 2009年27卷第1期页码：92-99
- Affiliations：
  
  Laboratory of Marine Genetics and Breeding Ocean University of China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30600455) and the National Basic Research Program of China (973 Program, No.2004CB117402)
- DOI：10.1007/s00343-009-0092-1
  中图分类号：
- 网络首发：2009-03-27，
  
  纸质出版：2009
- Accepted：
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Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus[J]. 海洋湖沼学报（英文）, 2009,27(1):92-99.

Chen, Y., Zhang, Q., Qi, J. et al. Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus, ., Chin. J. Ocean. Limnol. 27, 92–99 (2009). https://doi.org/10.1007/s00343-009-0092-1
Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus[J]. 海洋湖沼学报（英文）, 2009,27(1):92-99. DOI： 10.1007/s00343-009-0092-1.

Chen, Y., Zhang, Q., Qi, J. et al. Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus, ., Chin. J. Ocean. Limnol. 27, 92–99 (2009). https://doi.org/10.1007/s00343-009-0092-1 DOI：

摘要

Abstract

Flatfish or flounder moves one eye to change body proportion into vertebral asymmetry during metamorphosis

during which some become sinistral while others dextral. However

the mechanism behinds the eye-position has not been well understood. In this research

hybrids between Japanese flounder(♀) and stone flounder (♂) show mixed eye-location in both dextral type and sinistral type

and thus become good samples for studying the eye-migration. mRNAs from pro-metamorphosis sinistral and dextral hybrids larvae were screened with classical differential display RT-PCR (DD-RT-PCR) and representational difference analysis of cDNA (cDNA-RDA); 30 and 47 putative fragments were isolated

respectively. The cDNA fragments of creatine kinase and trypsinogen 2 precursor genes isolated by cDNA-RDA exhibited eye-position expression patterns during metamorphosis. However

none of the fragments was proved to be related to flatfishes’ eye-position specifically. Therefore

further studies and more sensitive gene isolated methods are needed to solve the problems.

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references

Ahlstrom, E. H., K. Amaoka, D. A. Hensley, H. G.. Moser and B. Y. Sumida, 1984. Pleuronectiformes: development ontogeny and systematics of fishes. Special publication 1. Am. Soc. Ichthyologists Herpetologists 1: 641–670.

Bao, B., G. Yang, Z. Liu, S. Li, Z. Wang and D. Ren, 2005. Isolation of SFRS3 gene and its differential expression during metamorphosis involving eye migration of Japanese flounder Paralichthys olivaceus. Biochim. Biophys. Acta 1725: 64–70.

Birkedal, R. and H. Gesser, 2003. Creatine kinase and mitochondrial respiration in hearts of trout, cod and freshwater turtle. J. Comp. Physiol. B 17: 493–499.

Hashimoto, H., M. Aritaki, K. Uozumi, S. Uji, T. Kurokawa and T. Suzuki, 2007. Embryogenesis and expression profiles of charon and nodal-pathway genes in sinistral (Paralichthys olivaceus) and dextral (Verasper variegatus) flounders. Zoolog. Sci. 24: 137–146.

Hashimoto, H., A. Mizuta, N. Okada, T. Suzuki, M. Tagawa, K. Tabata, Y. Yokoyama, M. Sakaguchi, M. Tanaka and H. Toyohara, 2002. Isolation and characterization of a Japanese flounder clonal line, reversed, which exhibits reversal of metamorphic left-right asymmetry. Mech. Dev. 111: 17–24.

Hildahl, J., M. Galay-Burgos, G.. Sweeney, I. E. Einarsdottir and B. T. Bjornsson, 2007. Identification of two isoforms of Atlantic halibut insulin-like growth factor-I receptor genes and quantitative gene expression during metamorphosis. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 147: 395–401.

Hubank, M. and D. G. Schatz, 1994. Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucleic Acids Res. 22: 5 640–5 648.

Inui, Y., T. Yamno and S. Miwa, 1995. The role of thyroid hormone in tissue development in metamorphosing flounder. Aquaculture 135: 87–98.

Liang, P. and A. B. Pardee, 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257: 967–971.

Lisitsyn, N. and M. Wigler, 1993. Cloning the differences between two complex genomes. Science 259: 946–951.

Minami, T., 1982. The early life history of a flounder Paralichthys olivaceus (in Japanese). Bull. Jpn. Soc. Sci. Fish. 48: 1 581–1 588.

Miwa, S. and Y. Inui, 1987. Effects of various doses of thyroxine and triiodothyronine on the metamorphosis of flounder (Paralichthys olivaceus). Gen. Comp. Endocrinol. 67: 356–363.

Miwa, S., M. Tagawa, Y. Inui and T. Hirano, 1988. Thyroxine surge in metamorphosing flounder larvae. Gen. Comp. Endocrinol. 70: 158–163.

Nordqvist, K. and V. Tohonen, 1997. An mRNA differential display strategy for cloning genes expressed during mouse gonad development. Int. J. Dev. Biol. 41: 627–638.

O’Neill, M. J. and A. H. Sinclair, 1997. Isolation of rare transcripts by representational difference analysis. Nucleic Acids Res. 25: 2 681–2 682.

Okada, N., Y. Takagi, T. Seikai, M. Tanaka and M. Tagawa, 2001. Asymmetrical development of bones and soft tissues during eye migration of metamorphosing Japanese flounder, Paralichthys olivaceus. Cell Tissue Res. 304: 59–66.

Seikai, T., J. B. Tanangonan and M. Tanaka, 1986. Temperature influence on larval growth and metamorphosis of the Japanese flounder Paralichthys olivaceus in the laboratory. Bull. Jap. Soc. Sci. Fish. 52(6): 983–994.

Tagawa, M. and M. Aritaki, 2005. Production of symmetrical flatfish by controlling the timing of thyroid hormone treatment in spotted halibut Verasper variegatus. Gen. Comp. Endocrinol. 141: 184–189.

Wang, Z., X. Wang and Q. Zhang, 2003. Interfamilial hybridization between Paralichthys olivaceus and Kareius bicoloratus — Early development and growth characters. Journal of Fishery Sciences of China 10: 30–37.

Yamano, K. and S. Miwa, 1998. Differential gene expression of thyroid hormone receptor alpha and beta in fish development. Gen. Comp. Endocrinol. 109: 75–85.

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