Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis

Li Li; Ximing Guo; Guofan Zhang

doi:10.1007/s00343-009-0074-3

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Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis

OriginalPaper | Updated：2023-05-16

- Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis
- Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis
- 海洋湖沼学报（英文） 2009年27卷第1期页码：74-79
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. Haskin Shellfish Research Laboratory Institute of Coastal and Marine Sciences Rutgers University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (NO.40730845, 39825121)
- DOI：10.1007/s00343-009-0074-3
  中图分类号：
- 网络首发：2009-03-27，
  
  纸质出版：2009
- Accepted：
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Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis[J]. 海洋湖沼学报（英文）, 2009,27(1):74-79.

Li, L., Guo, X. & Zhang, G. Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis., Chin. J. Ocean. Limnol. 27, 74–79 (2009). https://doi.org/10.1007/s00343-009-0074-3
Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis[J]. 海洋湖沼学报（英文）, 2009,27(1):74-79. DOI： 10.1007/s00343-009-0074-3.

Li, L., Guo, X. & Zhang, G. Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis., Chin. J. Ocean. Limnol. 27, 74–79 (2009). https://doi.org/10.1007/s00343-009-0074-3 DOI：

摘要

Abstract

Microsatellites were screened in a backcross family of the Pacific oyster

Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci

accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci

has been proved a fast

precise

and reliable method in microsatellite genotyping.

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references

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