Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein

Xuguang Hong; Xiuqin Sun; Minggang Zheng; Lingyun Qu; Jindong Zan; Jinxing Zhang

doi:10.1007/s00343-008-0375-y

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Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein

OriginalPaper | Updated：2023-05-16

- Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein
- Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein
- 海洋湖沼学报（英文） 2008年26卷第4期页码：375-379
- Affiliations：
  
  1. College of Life Science and Technology Ocean University of China
  2. First Institute of Oceanography SOA
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 program)(No. 2006AA100309)
- DOI：10.1007/s00343-008-0375-y
  中图分类号：
- 网络首发：2009-01-23，
  
  纸质出版：2008
- Accepted：
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Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein[J]. 海洋湖沼学报（英文）, 2008,26(4):375-379.

Hong, X., Sun, X., Zheng, M. et al. Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein., Chin. J. Ocean. Limnol. 26, 375–379 (2008). https://doi.org/10.1007/s00343-008-0375-y
Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein[J]. 海洋湖沼学报（英文）, 2008,26(4):375-379. DOI： 10.1007/s00343-008-0375-y.

Hong, X., Sun, X., Zheng, M. et al. Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein., Chin. J. Ocean. Limnol. 26, 375–379 (2008). https://doi.org/10.1007/s00343-008-0375-y DOI：

摘要

Abstract

Defensin is one of preserved ancient host defensive materials formed in biological evolution. As a regulator and effector molecule

it is very important in animals’ acquired immune system. This paper reports the defensin gene from the mixed liver and kidney cDNA library of abalone Haliotis discus hannai Ino. Sequence analysis shows that the gene sequence of full-length cDNA encodes 42 mature peptides (including six Cys)

molecular weight of 4 323 Da

and pI of 8.02. Amino acid sequence homology analysis shows that the peptides are highly similar (70% in common) to other insects defensin. Because of a typical insect-defensin structural character of mature peptide in the secondary structure

the polypeptide named Haliotis discus defensin (hd-def)

a novel of antimicrobial peptides

belongs to insects defensin subfamily. The RT-PCR result of Haliotis discus defensin shows that the gene can be expressed only in the hepatopancreas by Gram-negative and positive bacteria stimulation

which is ascribed to inducible expression. Therefore

it is revealed that the Haliotis discus defensin gene expression was related to the antibacterial infection of Haliotis discus hannai Ino.

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references

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