Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus

Wen Luo; Yunlong Zhao; Zhongliang Zhou; Chuanguang An; Qiang Ma

doi:10.1007/s00343-008-0062-z

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Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus

OriginalPaper | Updated：2023-05-16

- Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus
- Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus
- 海洋湖沼学报（英文） 2008年26卷第1期页码：62-68
- Affiliations：
  
  1. School of Life Science College of Shaoxing Arts and Sciences
  2. School of Life Science East China Normal University
- Author bio：
- Funds：
  
  Supported by the NNSF of China (No.30670227) and Shanghai Agricultural Science & Technology Key Grant [6-1(2006)].
- DOI：10.1007/s00343-008-0062-z
  中图分类号：
- 纸质出版：2008
- Accepted：
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Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus[J]. 海洋湖沼学报（英文）, 2008,26(1):62-68.

Luo, W., Zhao, Y., Zhou, Z. et al. Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus, ., Chin. J. Ocean. Limnol. 26, 62–68 (2008). https://doi.org/10.1007/s00343-008-0062-z
Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus[J]. 海洋湖沼学报（英文）, 2008,26(1):62-68. DOI： 10.1007/s00343-008-0062-z.

Luo, W., Zhao, Y., Zhou, Z. et al. Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus, ., Chin. J. Ocean. Limnol. 26, 62–68 (2008). https://doi.org/10.1007/s00343-008-0062-z DOI：

摘要

Abstract

The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ—PCR) methods. The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However

chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity

but also the regulatory mechanism for expression of trypsin gene to a certain degree.

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references

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