Cloning and sequence analysis of a full-length cDNA of SmPP1cb encoding turbot protein phosphatase 1 beta catalytic subunit

Fei Qi; Huarong Guo; Jian Wang

doi:10.1007/s00343-008-0054-z

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Cloning and sequence analysis of a full-length cDNA of SmPP1cb encoding turbot protein phosphatase 1 beta catalytic subunit

OriginalPaper | Updated：2023-05-16

- Cloning and sequence analysis of a full-length cDNA of SmPP1cb encoding turbot protein phosphatase 1 beta catalytic subunit
- Cloning and sequence analysis of a full-length cDNA of SmPP1cb encoding turbot protein phosphatase 1 beta catalytic subunit
- 海洋湖沼学报（英文） 2008年26卷第1期页码：54-61
- Affiliations：
  
  Ocean University of China
- Author bio：
- Funds：
  
  Supported by the 863 Program of China (No. AA2001628130)
- DOI：10.1007/s00343-008-0054-z
  中图分类号：
- 纸质出版：2008
- Accepted：
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Cloning and sequence analysis of a full-length cDNA of SmPP1cb encoding turbot protein phosphatase 1 beta catalytic subunit[J]. 海洋湖沼学报（英文）, 2008,26(1):54-61.

Qi, F., Guo, H. & Wang, J. Cloning and sequence analysis of a full-length cDNA of SmPP1cb encoding turbot protein phosphatase 1 beta catalytic subunit., Chin. J. Ocean. Limnol. 26, 54–61 (2008). https://doi.org/10.1007/s00343-008-0054-z
Cloning and sequence analysis of a full-length cDNA of SmPP1cb encoding turbot protein phosphatase 1 beta catalytic subunit[J]. 海洋湖沼学报（英文）, 2008,26(1):54-61. DOI： 10.1007/s00343-008-0054-z.

Qi, F., Guo, H. & Wang, J. Cloning and sequence analysis of a full-length cDNA of SmPP1cb encoding turbot protein phosphatase 1 beta catalytic subunit., Chin. J. Ocean. Limnol. 26, 54–61 (2008). https://doi.org/10.1007/s00343-008-0054-z DOI：

摘要

Abstract

Reversible protein phosphorylation

catalyzed by protein kinases and phosphatases

is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 (PP1) is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study

a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase 1(PP1cb)

was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus

designated SmPP1cb

by the rapid amplification of cDNA ends (RACE) technique. The cDNA sequence of SmPP1cb we obtained contains a 984 bp open reading frame (ORF)

flanked by a complete 39 bp 5′ untranslated region and 462 bp 3′ untranslated region. The ORF encodes a putative 327 amino acid protein

and the N-terminal section of this protein is highly acidic

Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp

a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B (PP2B). And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that

SmPP1cb is extremely conserved in both amino acid and nucleotide acid levels compared with the PP1cb of other vertebrates and invertebrates

and its Kozak motif contained in the 5′UTR around ATG start codon is GXXAXXGXXATGG

which is different from mammalian in two positions A−6 and G−3

indicating the possibility of different initiation of translation in turbot

and also the 3′UTR of SmPP1cb is highly diverse in the sequence similarity and length compared with other animals

especially zebrafish. The cloning and sequencing of SmPP1cb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.

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references

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