Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)

Zhiwen Zhang; Xiuqin Sun; Jinxing Zhang; Jindong Zan

doi:10.1007/s00343-008-0050-3

Your Location：

Home >

Browse articles >

Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)

OriginalPaper | Updated：2023-05-16

- Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)
- Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)
- 海洋湖沼学报（英文） 2008年26卷第1期页码：50-53
- Affiliations：
  
  1. College of Marine Life Sciences Ocean University of China
  2. The First Institute of Oceanography State Oceanic Administration of China
- Author bio：
- Funds：
  
  Supported by the Project under Major State Basic Research of China (No. G199901205)
- DOI：10.1007/s00343-008-0050-3
  中图分类号：
- 纸质出版：2008
- Accepted：
Scan QR Code
Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)[J]. 海洋湖沼学报（英文）, 2008,26(1):50-53.

Zhang, Z., Sun, X., Zhang, J. et al. Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)., Chin. J. Ocean. Limnol. 26, 50–53 (2008). https://doi.org/10.1007/s00343-008-0050-3
Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)[J]. 海洋湖沼学报（英文）, 2008,26(1):50-53. DOI： 10.1007/s00343-008-0050-3.

Zhang, Z., Sun, X., Zhang, J. et al. Cloning and expression of prion protein encoding gene of flounder (Paralichthys olivaceus)., Chin. J. Ocean. Limnol. 26, 50–53 (2008). https://doi.org/10.1007/s00343-008-0050-3 DOI：

摘要

Abstract

The prion protein (PrP) encoding gene of flounder (Paralichthys olivaceus) was cloned. It was not interrupted by an intron. This gene has two promoters in its 5′ upstream

indicating that its transcription may be intensive

and should have an important function. It was expressed in all 14 tissues tested

demonstrating that it is a house-keeping gene. Its expression in digestion and reproduction systems implies that the possible prions of fish may transfer horizontally.

关键词

Keywords

references

Asahida, T., T. O. Kokayashi, K. O. Saitoh, I. Nakayama, 1996. Tissue preservation and total DNA extraction from fish store at ambient temperature using buffers containing high concentration of urea. Fisheries Science 62: 727–730.

Funke-Kaiser, H, S. Theis, T. Behrouzi, et al., 2001. Functional characterization of the human prion protein promoter in neuronal and endothelial cells. J. Mol. Med. 79(9): 529–35.

Garcia-Crespo, D., R. A. Juste, A. Hurtado, 2005. Selection of ovine housekeeping genes for normalisation by real-time RT-PCR: analysis of PrP gene expression and genetic susceptibility to scrapie. BMC Vet Res. 28: 1–3.

Lee, I. Y., Westaway, D., Smit, A. F., Wang, K., Seto, J., Chen, L., Acharya, C., Ankener, M., Baskin, D., Cooper, C., Yao, H., Prusiner, S. B., and Hood, L. E., 1998. Complete genomic sequence and analysis of the prion protein gene region from three mammalian species. Genome Res. 8: 1 022–1 037.

Liao, M., Z. Zhang, G. Yang, 2005. Cloning and characterization of prion protein coding genes of Japanese seabass (Lateolabrax japonicus) and Japanese flounder (Paralichthys olivaceus). Aquaculture 249: 47–53.

Miller, M. W., E. S. Williams, 2003. Prion disease: horizontal prion transmission in mule deer. Nature 425(6953): 35–36.

Oidtmann, B., D. Simon, N. Holtkamp et al., 2003. Identification of cDNAs from Japanese pufferfish (Fugu rubripes) and Atlantic salmon (Salmo salar) coding for homologues to tetrapod prion proteins. FEBS Lett. 53: 96–100.

Premzl, M., J. E. Gready, L. S. Jermiin et al., 2004. Evolution of vertebrate genes related to prion and Shadoo proteins—clues from comparative genomic analysis. Mol. Biol. Evol. 21: 2 210–2 231.

Rivera-Milla, E., C. A. Stuermer, E. Malaga-Trillo, 2003. An evolutionary basis for scrapie disease: identification of a fish prion mRNA. Trends Genet. 19(2): 72–75.

Sander, P., H. Hamann, C. Drogemuller et al., 2005. Bovine prion protein gene (PRNP) promoter polymorphisms modulate PRNP expression and may be responsible for differences in bovine spongiform encephalopathy susceptibility. J. Biol. Chem. 280(45): 37 408–37 414.

Shaked, Y., H. Rosenmann, G. Talmor et al., 1999. A C-terminal-truncated PrP isoform is present in mature sperm. J. Biol. Chem. 274(45): 32 153–32 158.

Simonic T, S. Duga, B. Strumbo, R. Asselta, et al., 2000, cDNA cloning of turtle prion protein. FEBS Lett. 469(1): 33–38.

Zhang, L., N. Li, Q. G. Wang, B. L. Fan et al., 2002. Cloning and sequencing of quail and pigeon prion genes. Anim. Biotechnol. 13(1): 159–162.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

cDNA cloning and expression of a collectin from red-spotted grouper (Epinephelus akaara)

The role of Smad6 in immunity of the pearl oyster Pinctada fucata martensii

Cloning of catalase gene and antioxidant genes in Scophthalmus maximus response to metalloprotease of Vibrio anguillarum stress

Triterpenoid saponin biosynthesis genes and their expression patterns during the development of sea cucumber Apostichopus japonicus

Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus