Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)

Jindong Zan; Xiuqin Sun; Zhiwen Zhang; Lingyun Qu; Jinxing Zhang

doi:10.1007/s00343-007-0418-9

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Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)

OriginalPaper | Updated：2023-05-16

- Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)
- Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)
- 海洋湖沼学报（英文） 2007年25卷第4期页码：418-422
- Affiliations：
  
  First institute of Oceanography SOA
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program, No. 2003AA622030, 2006AA100309).
- DOI：10.1007/s00343-007-0418-9
  中图分类号：
- 纸质出版：2007
- Accepted：
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Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)[J]. 海洋湖沼学报（英文）, 2007,25(4):418-422.

Zan, J., Sun, X., Zhang, Z. et al. Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)., Chin. J. Ocean. Limnol. 25, 418–422 (2007). https://doi.org/10.1007/s00343-007-0418-9
Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)[J]. 海洋湖沼学报（英文）, 2007,25(4):418-422. DOI： 10.1007/s00343-007-0418-9.

Zan, J., Sun, X., Zhang, Z. et al. Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)., Chin. J. Ocean. Limnol. 25, 418–422 (2007). https://doi.org/10.1007/s00343-007-0418-9 DOI：

摘要

Abstract

Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn)

which has a wide range of hosts. Based on competitive quantitative PCR technology

we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106 virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104 virions/ml. No virus was found in the negative samples of flounder.

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references

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