Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus

Kui Chen; Xiaohang Huang; Yingmei Chai; Herbert Y. Gaisano

doi:10.1007/s00343-007-0378-0

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Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus

OriginalPaper | Updated：2023-05-16

- Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus
- Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus
- 海洋湖沼学报（英文） 2007年25卷第4期页码：378-385
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. The First Institute of Oceanography State Oceanic Administration
  3. Graduate University Chinese Academy of Sciences
  4. Department of Physiology University of Toronto
- Author bio：
- Funds：
  
  Supported by the NSFC (No.40476060) and Hi-Tech Research and Development Program of China (No. 2002AA629120).
- DOI：10.1007/s00343-007-0378-0
  中图分类号：
- 纸质出版：2007
- Accepted：
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Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus[J]. 海洋湖沼学报（英文）, 2007,25(4):378-385.

Chen, K., Huang, X., Chai, Y. et al. Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus, ., Chin. J. Ocean. Limnol. 25, 378–385 (2007). https://doi.org/10.1007/s00343-007-0378-0
Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus[J]. 海洋湖沼学报（英文）, 2007,25(4):378-385. DOI： 10.1007/s00343-007-0378-0.

Chen, K., Huang, X., Chai, Y. et al. Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus, ., Chin. J. Ocean. Limnol. 25, 378–385 (2007). https://doi.org/10.1007/s00343-007-0378-0 DOI：

摘要

Abstract

The t-SNARE protein SNAP-25 (synaptosome-associated protein of 25 kDa) plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene

the first step in the functional study of SNARE proteins in marine teleostean

was to obtain the cDNA of sea perch SNAP-25 (SPsn25) by RT-PCR and RACE-PCR amplification of a Japanese sea perch. The full-length cDNA of 831bp contains a CDS of 615 bp

coding 204 amino acid residues

and a 5 UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch had been identified through semi-quantitative RT-PCR and Western Blot assay. Together

these data again confirmed the nerve tissue specificity of the fish SNAP-25 gene expression.

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references

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