Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins

Hongming Ma; Kangsen Mai; Zhiguo Liufu; Wei Xu

doi:10.1007/s00343-007-0304-5

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Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins

OriginalPaper | Updated：2023-05-16

- Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins
- Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins
- 海洋湖沼学报（英文） 2007年25卷第3期页码：304-309
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Life Science and Technology Division Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program, No. G1999012012) and the Key Laboratory of Mariculture, Ministry of Education (No. 2004024)
- DOI：10.1007/s00343-007-0304-5
  中图分类号：
- 纸质出版：2007
- Accepted：
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Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins[J]. 海洋湖沼学报（英文）, 2007,25(3):304-309.

Ma, H., Mai, K., Liufu, Z. et al. Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins., Chin. J. Ocean. Limnol. 25, 304–309 (2007). https://doi.org/10.1007/s00343-007-0304-5
Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins[J]. 海洋湖沼学报（英文）, 2007,25(3):304-309. DOI： 10.1007/s00343-007-0304-5.

Ma, H., Mai, K., Liufu, Z. et al. Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins., Chin. J. Ocean. Limnol. 25, 304–309 (2007). https://doi.org/10.1007/s00343-007-0304-5 DOI：

摘要

Abstract

An actingene (CfACTI) was cloned by using RT-PCR

3’ and 5’RACE from hemocytes of the sea scallop Chlamys farreri. The full length of the transcript is 1 535 bp

which contains a long 3’ un-translated region of 436bp and 59bp of a 5’ un-translated sequence. The open reading frame encodes a polypeptide of 376 amino acids. Sequence comparisons indicated that CfACTI is more closely related to vertebrate cytoplasmic actins than muscle types. Phylogenetic analysis showed that molluscan actins could be generally divided into two categories: muscle and cytoplasmic

although both are similar to vertebrate cytoplasmic actins. It was also inferred that different isotypes existed in muscle or cytoplasma in mollusks. The genomic sequence of CfACTI was cloned and sequenced. Only one intron was detected: it was located between codons 42 and 43 and different from vertebrate actin genes.

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references

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