A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01

Dazhi Wang; Shugang Zhang; Huasheng Hong

doi:10.1007/s00343-007-0227-1

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A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01

OriginalPaper | Updated：2023-05-16

- A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01
- A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01
- 海洋湖沼学报（英文） 2007年25卷第2期页码：227-234
- Affiliations：
  
  State Key Laboratory of Marine Environmental Science/Environmental Science Research Center Xiamen University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No.40376032) and the Ministry of Science and Technology of the People’s Republic of China (No.2001CB409700).
- DOI：10.1007/s00343-007-0227-1
  中图分类号：
- 纸质出版：2007
- Accepted：
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A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01[J]. 海洋湖沼学报（英文）, 2007,25(2):227-234.

Wang, D., Zhang, S. & Hong, H. A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01., Chin. J. Ocean. Limnol. 25, 227–234 (2007). https://doi.org/10.1007/s00343-007-0227-1
A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01[J]. 海洋湖沼学报（英文）, 2007,25(2):227-234. DOI： 10.1007/s00343-007-0227-1.

Wang, D., Zhang, S. & Hong, H. A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01., Chin. J. Ocean. Limnol. 25, 227–234 (2007). https://doi.org/10.1007/s00343-007-0227-1 DOI：

摘要

Abstract

Sulfotransferase (ST) is the first enzyme discovered in association with paralytic shellfish poisoning (PSP) toxin biosynthesis in toxic dinoflagellates. This study investigates the ST activity in crude enzyme extraction of a toxic dinoflagellate species

Alexandrium tamarense CI01. The results show that crude enzyme can transfer a sulfate group from 3′-phosphoadenosine 5′-phosphosulfate (PAPS) to N-21 in the carbamoyl group of gonyautoxin 2/3 (GTX2/3) to produce C1/C2

but is inactive toward STX to produce GTX5. The crude enzyme is optimally active at pH 6.0 and 15°C. The activity is enhanced by Co2+

Mg2+

Mn2+ and Ca2+ individually

but is inhibited by Cu2+. Moreover

the activity shows no difference when various sulfur compounds are used as sulfate donors. These results demonstrate that the ST specific to GTX2/3 is present in the cells of A. tamarense CI01 and is involved in PSP toxin biosynthesis. In addition

the ST from different dinoflagellates is species-specific

which explains well the various biosynthesis pathways of the PSP toxins in toxic dinoflagellates.

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Keywords

references

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