Genetic study of kelp “901” strain

Peng Xia; Xiuliang Wang; Xiaojie Li; Yushan Zhao; Lin Yao; Delin Duan

doi:10.1007/BF02894231

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Genetic study of kelp “901” strain

OriginalPaper | Updated：2023-05-16

- Genetic study of kelp “901” strain
- Genetic study of kelp “901” strain
- 海洋湖沼学报（英文） 2005年23卷第2期页码：152-157
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School of Chinese Academy of Sciences
  3. Yantai Oriental Ocean Development Co. Ltd
  4. Qingdao University
- Author bio：
- Funds：
  
  Financially supported by 863 program of China (No.2001AA621090)
- DOI：10.1007/BF02894231
  中图分类号：
- 纸质出版：2005
- Accepted：
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Genetic study of kelp “901” strain[J]. 海洋湖沼学报（英文）, 2005,23(2):152-157.

Peng, X., Xiuliang, W., Xiaojie, L. et al. Genetic study of kelp “901” strain., Chin. J. Ocean. Limnol. 23, 152–157 (2005). https://doi.org/10.1007/BF02894231
Genetic study of kelp “901” strain[J]. 海洋湖沼学报（英文）, 2005,23(2):152-157. DOI： 10.1007/BF02894231.

Peng, X., Xiuliang, W., Xiaojie, L. et al. Genetic study of kelp “901” strain., Chin. J. Ocean. Limnol. 23, 152–157 (2005). https://doi.org/10.1007/BF02894231 DOI：

摘要

Abstract

Based on DNA extraction and optimization of random amplified reaction (RAPD) to the gametophytes and sporophytes of Kelp “901” strain

genetic study on variation was conducted to its parents and offsprings of F6

and F9 generation. RAPD results have shown that among 30 selected primers for gametophytes

297 loci ranging from 200 to 3 000 bp were obtained in the average of 9.9 loci for each primer. This indicated a high polymorphic rate with RAPD detection. UPGMA (unweighted pair-group method arithmetic average) analysis showed that each male and female gametophyte of a generation could be clustered into one pair separately. The genetic distances of the Kelp 901 generation were 0.3212–0.4767

and the maximum was between F7 and F8 (0.4767). Identity analysis showed that F6 generation was more close to the female parent (0.6593)

and F7 generation was more close to the male parent (0.578 8). To the sporophytes study in 24 selected primers for RAPD amplification

191 loci ranging from 230–2800 bp were obtained

in the average to each primer of 8.0 loci. The heterozygosity to six populations were male parent (0.2239)

female parent (0.1072)

F6 (0.2164)

F7(0.2286)

F8(0.2296) and F9(0.3172). The nearest genetic distance was 0.083 5(F8

F9). Total heterozygosity (HT) of F6

F8 and F9 generations was 0.3186

the average heterozygosity (HS) for F6

F8 and F9 generations was 0.2480

and deduced coefficient of population differentiation (Gst) was 22.2%. Six sequence characterized amplified regions (SCAR) were preliminary screened through RAPD analysis. It needed to be verified in detail as they are significant for molecular marker assistance in breeding and selectingLaminaria.

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Keywords

references

Billot, C., S. Boury, H. Benet and B. Kloareg, 1999. Development of RAPD marker for parentage analysis inLaminaria digitata.Botanica Marina 42: 307–314.

Bolton, J. J., I. Germann and K. Luning, 1983. Hybridization between Altanic and Pacific representatives of the simplices section ofLaminaria (Phaeophyta).Phycologia 22(2): 131–140.

Chapman, A. R. O., 1974. The genetic basis of morphological differentiation in someLaminaria populations.Marine Biology 24: 85–91.

Dutcher, J. A. and D. F. Kapraun, 1994. Random amplified polymorphic DNA (RAPD) identification of genetic variation in three species ofPorphyra (Bangiales, Rhodophyta).J. Appl. Phycol. 6: 267–273

Dudley, J. W., M. A. Saghai and G. K. Rufener, 1991. Molecular markers and grouping of parents in maize breeding programs.Crop Science 31: 718–723.

Fang, T. C., C. Y. Wu, B. Y. Jiang, J. J. Li, and K. Z. Ren, 1962. The breeding of a new breed of Haidai (Laminaria japonica Aresch.) and its preliminary genetic analysis.Acta Botanica Sinica 10(3): 197–209. (in Chinese with English abstract)

Fang, T. C., B. Y. Jiang and J. J. Li, 1965. Further studies of the genetics ofLaminaria frond-length.Oceanologia et Limnologia Sinica 7(1): 59–66. (in Chinese with English abstract)

Fang, T. C., B. Y. Jiang and J. J. Li, 1962. On the inheritance of stipe length in Haidai (Laminaria japonica Aresch.).Acta Botanica Sinica 10(4): 327–335. (in Chinese with English abstract)

Fain, S. R., L. D. Druehl and D. L. Baillie, 1988. Repeat and single copy sequences are differentially conserved in the evolution of kelp chloroplast DNA.J. Phycol 24: 292–302.

He, Y. J., Y. P. Zou, X. D. Wang, Z. G. Zheng, D. M. Zhang and D. L. Duan, 2003. Assessing the germplasm ofLaminaria (Phaeophyceae) with random amplified polymorphic DNA (RAPD) method.Chin. J. Oceanol. Limnol. 21(3): 141–148.

Ho, C. L., S. M. Phang and T. Pang, 1995. Molecular characterization ofSargassum polycystum andS. siliquosum (Phaeophyta) by polymerase chain reaction (PCR) using random amplified polymorphic DNA (RAPD) primers.J. Appl. Phycol. 7: 33–44

Luning, K., R. O. Chapman and K. H. Mann, 1978. Cross experiments in the non-digitate complex ofLaminaria from both sides of the Atlantic.Phycologia 17(3): 293–298.

Mathieson, A. C., T. A. Norton and M. Neushul, 1981. The taxonomic implications of genetic and environmentally induced variations in seaweeds morphology.Botanical Review 47: 313–347.

Milbourne, D., R. Meyer and J. E. Bradshaw, 1997. Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato.Molecular Breeding. 3: 127–136.

Patwary, M. U. and J. P. van der Meer, 1994. Application of RAPD markers in an examination of heterosis inGelidium vagum (Rhodophyta).J. Phycol. 30: 91–97.

Tseng, C. K., 1986.Laminaria mariculture in China.In: Doty, M. S., Caddy, J. F. eds., Santelices case studies of seven commercial seaweed resources. FAO Fisheries technical paper. p. 281, 239–263.

tom Dieck (Bartsch), I. and E. C. de Oliveria, 1993. The section digitatae of the genusLaminaria (Phaeophyta) in the northern and southern Altanic: crossing experiments and temperature response.Marine Biology 115: 151–160.

Zhang, Q. S., S. P. Liu, S. C. Qu, Z. P. Tian, Z. M. Guo, Y. Z. Cong and Z. L. Li, 2001. Studies on rearing new variety ofKelp “901”.Transactions of Oceanology and Limnology 2: 46–53. (in Chinese with English abstract)

Zhang, J. Y. and Z. X. Fang, 1980. Preliminary observations on the inheritance of frond thickness inLaminaria japonica.Acta Genetica Sinica 7(3): 257–262. (in Chinese with English abstract)

Wang, X. L., Y. X. Yang, Y. Z. Cong and D. L. Duan, 2004. DNA fingerprinting of selectedLaminaria (Phaeophyta) gametophytes by RAPD markers.Aquaculture 238: 143–153.

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