Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa

Fan Tingjun; Jin Lingyun; Wang Xiaofeng

doi:10.1007/BF02860424

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Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa

OriginalPaper | Updated：2023-05-16

- Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa
- Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa
- 海洋湖沼学报（英文） 2003年21卷第4期页码：305-311
- Affiliations：
  
  Department of Marine Biology College of Marine Life Sciences Ocean University of China
- Author bio：
- Funds：
  
  This work was partially supported and funded by the China Education Committee as the “Imbursement Project for Studied Abroad Returnees”.
- DOI：10.1007/BF02860424
  中图分类号：
- 网络首发：2013-01-31，
  
  纸质出版：2003
- Accepted：
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Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa[J]. 海洋湖沼学报（英文）, 2003,21(4):305-311.

Tingjun, F., Lingyun, J. & Xiaofeng, W. Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa, ., Chin. J. Ocean. Limnol. 21, 305–311 (2003). https://doi.org/10.1007/BF02860424
Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa[J]. 海洋湖沼学报（英文）, 2003,21(4):305-311. DOI： 10.1007/BF02860424.

Tingjun, F., Lingyun, J. & Xiaofeng, W. Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa, ., Chin. J. Ocean. Limnol. 21, 305–311 (2003). https://doi.org/10.1007/BF02860424 DOI：

摘要

Abstract

Effects of basic fibroblast growth factor (bFGF) and insulin-like growth factor II (IGF-II) on cartilage cells from proboscis of skate

Raja porasa Günther

were investigated in this study. The cartilage cells were cultured in 20% FBS-supplemented MEM medium at 24°C. Twelve hours after culture initiation

the cartilage cells were treated with bFGF and IGF-II at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF-II was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF-II together

revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF-II together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7.

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references

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