Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro

L. S. Dolmatova; M. G. Eliseykina; N. F. Timchenko; A. L. Kovaleva; O. A. Shitkova

doi:10.1007/BF02860423

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Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro

OriginalPaper | Updated：2023-05-16

- Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro
- Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro
- 海洋湖沼学报（英文） 2003年21卷第4期页码：293-304
- Affiliations：
  
  1. . I. Il'ichev Pacific Oceanological Institute,V
  2. Institute of Marine Biology
  3. Research Institute of Epidemiology and Microbiology
- Author bio：
- Funds：
  
  This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).
- DOI：10.1007/BF02860423
  中图分类号：
- 网络首发：2013-01-31，
  
  纸质出版：2003
- Accepted：
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Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro[J]. 海洋湖沼学报（英文）, 2003,21(4):293-304.

Dolmatova, L.S., Eliseykina, M.G., Timchenko, N.F. et al. Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro., Chin. J. Ocean. Limnol. 21, 293–304 (2003). https://doi.org/10.1007/BF02860423
Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro[J]. 海洋湖沼学报（英文）, 2003,21(4):293-304. DOI： 10.1007/BF02860423.

Dolmatova, L.S., Eliseykina, M.G., Timchenko, N.F. et al. Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro., Chin. J. Ocean. Limnol. 21, 293–304 (2003). https://doi.org/10.1007/BF02860423 DOI：

摘要

Abstract

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea

Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml

but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP

TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml

respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment

and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST

lectin stimulated ROS to lesser extent than that with lectin alone. When catalase

TST

and lectin were added into the FPMC simultaneously

ROS increase was similar to that under lectin treatment alone. On the whole

data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes

and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians.

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references

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