Isolation of plasmid from the blue-green algaSpirulina platensis

Qin Song; Tong Shun; Zhang Peijun; C. K. Tseng

doi:10.1007/BF02850862

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Isolation of plasmid from the blue-green algaSpirulina platensis

OriginalPaper | Updated：2023-05-16

- Isolation of plasmid from the blue-green algaSpirulina platensis
- Isolation of plasmid from the blue-green algaSpirulina platensis
- 海洋湖沼学报（英文） 1993年11卷第3期页码：285-288
- Affiliations：
  
  Experimental Marine Biology Laboratory Institute of Oceanology
- Author bio：
- Funds：
  
  Contribution No. 79 of the Experimental Marine Biology Laboratory and 2153 of the Institute of Oceanology, Academia Sinica.;This research was supported in part by The President of the Chinese Academy of Sciences Foundation.
- DOI：10.1007/BF02850862
  中图分类号：
- 纸质出版：1993
- Accepted：
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Isolation of plasmid from the blue-green algaSpirulina platensis[J]. 海洋湖沼学报（英文）, 1993,11(3):285-288.

Song, Q., Shun, T., Peijun, Z. et al. Isolation of plasmid from the blue-green algaSpirulina platensis, ., Chin. J. Ocean. Limnol. 11, 285–288 (1993). https://doi.org/10.1007/BF02850862
Isolation of plasmid from the blue-green algaSpirulina platensis[J]. 海洋湖沼学报（英文）, 1993,11(3):285-288. DOI： 10.1007/BF02850862.

Song, Q., Shun, T., Peijun, Z. et al. Isolation of plasmid from the blue-green algaSpirulina platensis, ., Chin. J. Ocean. Limnol. 11, 285–288 (1993). https://doi.org/10.1007/BF02850862 DOI：

摘要

Abstract

CCC plasmid was isolated from an economically important blue-green alga —Spirulina platensis (1.7×106 dalton from the S6 strain and 1.2×106 dalton from the F3 strain) using a rapid method based on ultrasonic disruption of algal cells and alkaline removal of chromosomal DNA. The difference in the molecular weight of the CCC DNAs from the two strains differing in form suggests that plasmid may be related with the differentiation of algal form. This modified method

which does not use any lysozyme

is a quick and effective method of plasmid isolation

especially for filamentous blue-green algae.

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references

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Hondel van den C. A. M. J. J., Keegstm, W., Borrias, W. E. et al., 1979. Homology of plasmids in strains of unicellular cyanobacteria.Plasmid 2: 323–333.

Lambert, G. R. and Carr, N. G., 1982. Rapid small-scale plasmid isolation by several methods from filamentous cyanobacteria.Arch Microbiol. 133: 122–125.

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Schwabe, W., Hubschman, T., Meixner, M., et al., 1990. Transcription andin vivo expression of aMicrocystis plasmid.Curr. Microbiol. 20: 365–368.

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Wolk, C. P. and Wojciuch, E., 1973. Simple methods for plating single vegetative cells of, and for replica plating filamentous blue green algae.Arch. Microbiol. 91: 91–95.

Zarrouk, C., 1966. Contribution al l’Etude d’une Cyanophycee Influence de Divers Facteurs Physiques et Chimiques Surlas Croissance et la Photosyntheses deSpirulina maxima. Thesis, Univ. Paris.

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