Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms

Liang Ying; Mai Kang-sen; Sun Shi-chun; Yu Dao-zhan

doi:10.1007/BF02850662

Your Location：

Home >

Browse articles >

Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms

OriginalPaper | Updated：2023-05-16

- Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms
- Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms
- 海洋湖沼学报（英文） 2001年19卷第3期页码：249-254
- Affiliations：
  
  The Key Laboratory of Mariculture Ministry of Education Ocean University of Qingdao
- Author bio：
- Funds：
  
  Project supported by the Hi-Tech “863” Programs of the China Ministry of Science and Technology (863-819-02-01).
- DOI：10.1007/BF02850662
  中图分类号：
- 纸质出版：2001
- Accepted：
Scan QR Code
Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms[J]. 海洋湖沼学报（英文）, 2001,19(3):249-254.

Ying, L., Kang-sen, M., Shi-chun, S. et al. Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms., Chin. J. Ocean. Limnol. 19, 249–254 (2001). https://doi.org/10.1007/BF02850662
Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms[J]. 海洋湖沼学报（英文）, 2001,19(3):249-254. DOI： 10.1007/BF02850662.

Ying, L., Kang-sen, M., Shi-chun, S. et al. Effect of light intensity on the total lipid and fatty acid composition of six strains of marine diatoms., Chin. J. Ocean. Limnol. 19, 249–254 (2001). https://doi.org/10.1007/BF02850662 DOI：

摘要

Abstract

The effect of light intensity (1500 lx and 5000 lx) on the total lipid and fatty acid composition of six strains of marine diatomsCylindrotheca fusiformis (B211)

Phaeodactylum tricornutum (B114

B118 and B221)Nitzschia closterium (B222) andChaetoceros gracilis (B13) was investigated. The total lipids of B13

B114

and B211 grown at 5000 lx were lower than those grown at 1500 lx. No evident changes were observed in B118

B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14∶0

16∶0

16∶1 (n−7) and 20∶5(n−3).Cylindrotheca fusiformis had high percentage of 20∶4n–6(9.2–10.9%). The total polyunsaturated fatty acid in all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains exceptChaetoceros gracilis.

关键词

Keywords

references

Alonso, D. L., Grima, E. M., Sanchez P. J. A. et al., 1992.Isolation of clones ofIsochrysis galbana rich in eicosapentaenoic acid.Aquaculture 102: 363–371.

Bligh, E. G., 1959. A rapid method of total lipid extraction and purification.Can. J. Biochem. Physio. 37: 911–917.

Brown, M. B., Dunstan, G. A., Norwood, S. J., et al., 1996. Effects of harvest stage and light on the biochemical composition of the diatomThalassiosira pseudonana.J. Phycol. 32: 64–73.

Chen Mingyao, 1995. Living Food Culture. Agriculture Publishing Company, Beijing, p. 65–71. (in Chinese)

Dunstan, G. A., Volkman, J. K., Barrett, S. M. et al., 1994. Essential polyunsaturated fatty acids from 14 species of diatom (Bacillariophyceae).Phytochemistry 35: 155–161.

Grima, E. M., Medina, A. R., Gimenez, A. G., et al., 1996. Gram—scale purification of eicosapentaenoic acid (EPA, 20∶5n−3) from wetPhaeodactylum tricornutum UTEX640 biomass.J. Appl. Phycol. 8: 359–367.

Kayama, M., Araki, S., Sato, S., 1989. Lipids of Marine Plants.In: Ackman, R. G. ed, Marine Biogenic Lipids, Fats, and Oils. Volume II, CRC Press, Inc. Boca Raton, Florida, p. 4–21.

Orcutt, D. M., Patterson, G. W., 1975. Sterol, fatty acid and elemental composition of diatoms grown in chemically defined media.Comp. Biochem. Physiol. 50B: 579–583.

Reitan, K. I., Rainuzzo, J. R., Olsen, Y., 1994. Effect of nutrient limitation on fatty acid and lipid content of marine microalgae,J. Phycol. 30: 972–979.

Renaud, S. M., Parry, D. L., Thinh, L. V., et al., 1991. Effect of light intensity on the proximate biochemical and fatty acid composition ofIsochrysis sp. andNannochloropsis oculata for use in tropical aquaculture.J. Appl. Phycol. 3: 43–53.

Seto, A., Wang, H. L., Hesseltine, C. W., 1984. Culture conditions affect eicosapentaenoic acid content ofChlorella minutissima.Jaocs 61(5): 892–894.

Sukenik, A., Carmeli, Y., 1990. Lipid synthesis and fatty acid composition inNannochloropsis sp. (Eustigmatophyceae) grown in a light-dark cycle.J. Phycol. 26: 463–469.

Tan, C. K., Johns, M. R., 1996. Screening of diatoms for heterotrophic eicosapentaenoic acid production.J. Appl. Phycol. 8: 59–64.

Teshima, S., Yamasaki, S., Kanazawa, A. et al., 1983. Effects of water temperature and salinity on eicosapentaenoic acid level of marine Chlorella.Bull. Jap. Soc. Sci. Fish. 49: 805.

Thompson, P. A., Harrison, P. J., Whyte, J. N. C., 1990. Influence of irradiance on the fatty acid composition of phytoplankton.J. Phycol. 26: 278–288.

Viso, A. C., Marty, J. C., 1993. Fatty acids from 28 marine microalgae.Phytochemistry 34: 1521–1533.

Volkman, J. K., Jeffrey, S. W., Nichols, P. D. et al., 1989. Fatty acid and lipid composition of 10 species of microalgae used in mariculture.J. Exp. Mar. Biol. Ecol 128: 219–240.

Yongmanitchai, W., Ward, O. P., 1989. ω-3 fatty acid. Alternative sources of production.Process Biochem. 8: 117–125.

Yongmanitchai, W., Ward, O. P., 1991. Growth and omega-3 fatty acid production byPhaeodactylum tricornutum under different culture conditions.Appl. Envir. Microbiol. 57: 419–425.

Zhou Hongqi, Renaud, S. M., Parry, D. L., 1996. Effect of temperature on growth, total lipid content and fatty acid composition of the microalgae,Nitzschia closterium, Nitzschia paleacea andPavlova sp.J. Fish. China 20: 235–240. (in Chinese)

Zhukova, N. V., Aizdaicher, N. A., 1995. Fatty acid composition of 15 species of marine microalgae.Phytochemistry 39: 351–356.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Silicon limitation affects diatom’s resistance to copepod grazing

Two new Stephanodiscaceae species (Bacillariophyta) from SW China

The Middle and Late Holocene climate change in Bohai Bay revealed by diatom proxy

Response of phytoplankton functional group to spring drought in a large subtropical reservoir

Lineaperpetua gen. nov.: a new diatom genus in the Thalassiosirales supported by morphology and molecular data