Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis

Gu Tian-qing; Zhang Hui-miao; Sun Shi-hua

doi:10.1007/BF02850536

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Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis

OriginalPaper | Updated：2023-05-16

- Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis
- Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis
- 海洋湖沼学报（英文） 1996年14卷第1期页码：27-30
- Affiliations：
  
  Institute of Botany
- Author bio：
- Funds：
  
  The research supported by the NSFC.
- DOI：10.1007/BF02850536
  中图分类号：
- 纸质出版：1996
- Accepted：
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Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis[J]. 海洋湖沼学报（英文）, 1996,14(1):27-30.

Tian-qing, G., Hui-miao, Z. & Shi-hua, S. Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis, ., Chin. J. Ocean. Limnol. 14, 27–30 (1996). https://doi.org/10.1007/BF02850536
Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis[J]. 海洋湖沼学报（英文）, 1996,14(1):27-30. DOI： 10.1007/BF02850536.

Tian-qing, G., Hui-miao, Z. & Shi-hua, S. Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis, ., Chin. J. Ocean. Limnol. 14, 27–30 (1996). https://doi.org/10.1007/BF02850536 DOI：

摘要

Abstract

A component (s-factor) with obvious promoting effect on hydrogen evolution of hydrogenase has been isolated and extracted from a cell-free preparation ofSpirulina platensis. The effect of the s-factor in the reaction system is similar to that of Na2S2O4

but is coupled with light. The s-factor has the maximum absorption peak at 620 nm in the oxidized state

at 590 nm in the reduced state. The partially purified s-factor showed two bands by SDS-PAGE and is distinctly different from phycocyanin

which has no change of oxidized state and reduced state absorption spectra

and also has no promoting effect on hydrogenase ofSpirulina platensis under the light.

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references

Gu Tianqing, Wang Fazhu, 1984. Studies on hydrogen bySpirulina, platensis.Proc. Int. Seaweed Symp. 11: 467–470.

Gu Tianqing, Zhou Peizhen, 1987. Isolation and characterization of hydrogenase fromSpirulina platensis.Hydrobiologia 151/152: 557–561.

Herrero A, Floes E., Guerrero M. G., 1981. Regulation of nitrate reductase levels in the cyanobacteriaAnacystis nidulans, Anabaena sp. strain 7119, andNostoc sp strain 6719.J. Bacteria 145: 175–180.

Laemmli U. K., 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4.Nature 227: 686.

Maria J. L., Juan L. S., Krishana K. R., et al., 1979. Isolation and characterization of the hydrogenase activity from the non-heterocystous cyanobacteriumSpirulina maxima.Febs Lett. 98: 342–346.

Pinevitch V. V., Verzilin N. N., Mikhailiv A. A., 1970.Spirulina platensis-a new object of intensity mass cultivation.Fiziol. Rast. 17: 1037–1045. (in Russian). English translation inSov. Pi. Physiol.,17: 866–873.

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