The induction and extraction of metallothioneins inArtemia

Chen Bing-liang; Paul K. Chien

doi:10.1007/BF02850516

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The induction and extraction of metallothioneins inArtemia

OriginalPaper | Updated：2023-05-16

- The induction and extraction of metallothioneins inArtemia
- The induction and extraction of metallothioneins inArtemia
- 海洋湖沼学报（英文） 1994年12卷第2期页码：175-179
- Affiliations：
  
  University of San Francisco
- Author bio：
- Funds：
- DOI：10.1007/BF02850516
  中图分类号：
- 纸质出版：1994
- Accepted：
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The induction and extraction of metallothioneins inArtemia[J]. 海洋湖沼学报（英文）, 1994,12(2):175-179.

Bing-liang, C., Chien, P.K. The induction and extraction of metallothioneins inArtemia, ., Chin. J. Ocean. Limnol. 12, 175–179 (1994). https://doi.org/10.1007/BF02850516
The induction and extraction of metallothioneins inArtemia[J]. 海洋湖沼学报（英文）, 1994,12(2):175-179. DOI： 10.1007/BF02850516.

Bing-liang, C., Chien, P.K. The induction and extraction of metallothioneins inArtemia, ., Chin. J. Ocean. Limnol. 12, 175–179 (1994). https://doi.org/10.1007/BF02850516 DOI：

摘要

Abstract

A small amount of heavy metal binding protein

identified by BioRad Protein Assay

has been isolated from the adult brine shrimp

Artemia franciscanus. This protein has an apparent molecular weight between 6000 to 9000 dalton. a UV absorption peak at 260 instead of 280 nm like most proteins; and has high affinity towards binding with radioactive labeled109Cd. These characteristics are similar to that of metallothioneins reported for many vertebrate and invertebrate

marine and terrestrial animals. After the brine shrimp is exposed to a small amount of Cd2+ for 24 h

a large amount of metallothionein can be isolated

showing the inducibility of this detoxifying protein in the adultArtemia in a short period of time.

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references

Acey, R. A., Yoshida, B. N. and Edep, M. E., 1989. Metalloproteins in DevelopingArtemia, In: Cell and Molecular Biology ofArtenia Development, Plenum Press, pp. 203–219.

Frankenne, F., Noel-Lambat, F. and Disteche, A., 1980. Isolation and characterization of metallothionein from cadmium-loaded musselMytilus edulis.Comp. Biochem. Physiol.,66(C): 179–182.

Furst, A. and Nguyen, Q., 1989. Cadmium-induced metallothionein in the Earthworm (Lumbricus terrestris).Biological Trace Element Research 21: 81–85.

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Nemer, M., Tavaglini, E. C., Rondinelli, E. et al., 1984. Developmental egulation, induction, and emdryonic tissue specificity of Sea urchin metallothionein gene expression.Developmental Biology 102: 471–482.

Olafson, R. W., Kearns, A. and Sim, R. G., 1979. Heavy metal induction of metallothiorein synthesis in the hepatopancreas of the crabScylla serrata.Comp. Biochem. Physiol. 62(B): 417–424.

Olafson, R. W., Sim, R. G. and Boto, K. G., 1979. Isolation and chemical characterization of the heavy metal-binding protein metallothionein form marine invertebrates.Comp. Biochem. Physiol. 62(B): 407–416.

Overnell, J. and Trewhella, E., 1979. Evidence for the natural occurrence of (Cadmium, Copper) metallothionein in the crabCancer pagurus.Comp. Biochem. Physiol. 64(C): 69–76.

Rice, M. A. and Chien, P. K., 1979. Uptake, binding and clearance of divalent cadmium inGlycera dibranchiata (Annelida: Polychaeta).Marine Biology 53: 33–39.

Udom, A. O. and Brady, F. O., 1980. Reactivation vitro of zinc-requiring apo-enzymes by rat liver zinc-thionein.Biochem. J. 187: 329.

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