The reaction time of organ-forming substance in goldfish EGG and its relationship with mesodermal formation

Zhang Shicui; Wu Shangqin

doi:10.1007/BF02850452

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The reaction time of organ-forming substance in goldfish EGG and its relationship with mesodermal formation

OriginalPaper | Updated：2023-05-16

- The reaction time of organ-forming substance in goldfish EGG and its relationship with mesodermal formation
- The reaction time of organ-forming substance in goldfish EGG and its relationship with mesodermal formation
- 海洋湖沼学报（英文） 1988年6卷第4期页码：283-289
- Affiliations：
  
  Institute of Oceanology
- Author bio：
- Funds：
  
  Contribution No. 1507 from the Institute of Oceanology, Academia Sinica
- DOI：10.1007/BF02850452
  中图分类号：
- 纸质出版：1988
- Accepted：
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The reaction time of organ-forming substance in goldfish EGG and its relationship with mesodermal formation[J]. 海洋湖沼学报（英文）, 1988,6(4):283-289.

Shicui, Z., Shangqin, W. The reaction time of organ-forming substance in goldfish EGG and its relationship with mesodermal formation., Chin. J. Ocean. Limnol. 6, 283–289 (1988). https://doi.org/10.1007/BF02850452
The reaction time of organ-forming substance in goldfish EGG and its relationship with mesodermal formation[J]. 海洋湖沼学报（英文）, 1988,6(4):283-289. DOI： 10.1007/BF02850452.

Shicui, Z., Shangqin, W. The reaction time of organ-forming substance in goldfish EGG and its relationship with mesodermal formation., Chin. J. Ocean. Limnol. 6, 283–289 (1988). https://doi.org/10.1007/BF02850452 DOI：

摘要

Abstract

Fertilized goldfish eggs were dechorionated with a pair of forceps and were cut off along or a little above the equator into animal and vegetative parts at desired stages with a glass needle or ligated into two connected fragments before cleavage with baby hair loop. Some of the ligated eggs were detached by further fastening soon after ligation

and some released later at different stages (2-cell

16-cell

128-cell

512-cell

mid-blastula) to let the organ-forming substance (OFS) enter the blastoderm. The cholinesterase (ChE) in the resulting embryos was assayed. The results are as follows. 1. All the 142 embryos developed from the animal hemispheres cut off or ligated off before cleavage gave rise to hyperblastula in which no ChE activity was observed. 2. All 50 embryos obtained from animal halves isolated at the 8-cell stage produced ChE. 3. Embryos developed from the eggs released before the 512-cell stage formed ChE

but the later the releasing of the hair knots

the smaller the number of ChE-producing embryos. 4. After the 512-cell stage (excluding this stage)

neither ChE nor tissue differentiation occurred in the embryos developed from the unfastened eggs though their OFS flow was set free. Since ChE is thought to be a muscle-specific enzyme in the early developmental stage

it is concluded that the OFS in goldfish egg appears to be indispensable for the establishment of the mesoderm.

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references

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