Purification and some properties of Fe protein of nitrogenase from

Du Daixian; Lin Huimin; He Zhenrong; Dai Lingfen; Xin Wusheng; Li Shanghao

doi:10.1007/BF02849684

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Purification and some properties of Fe protein of nitrogenase from

OriginalPaper | Updated：2023-05-16

- Purification and some properties of Fe protein of nitrogenase from
- Purification and some properties of Fe protein of nitrogenase from
- 海洋湖沼学报（英文） 1990年8卷第4期页码：378-384
- Affiliations：
  
  Institute of Hydrobiology
- Author bio：
- Funds：
- DOI：10.1007/BF02849684
  中图分类号：
- 纸质出版：1990
- Accepted：
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Purification and some properties of Fe protein of nitrogenase from[J]. 海洋湖沼学报（英文）, 1990,8(4):378-384.

Daixian, D., Huimin, L., Zhenrong, H. et al. Purification and some properties of Fe protein of nitrogenase from., Chin. J. Ocean. Limnol. 8, 378–384 (1990). https://doi.org/10.1007/BF02849684
Purification and some properties of Fe protein of nitrogenase from[J]. 海洋湖沼学报（英文）, 1990,8(4):378-384. DOI： 10.1007/BF02849684.

Daixian, D., Huimin, L., Zhenrong, H. et al. Purification and some properties of Fe protein of nitrogenase from., Chin. J. Ocean. Limnol. 8, 378–384 (1990). https://doi.org/10.1007/BF02849684 DOI：

摘要

Abstract

The Fe protein ofAnabaena cylindrica was first separated and purified by chromatography through DEAE-cellulose columns then by gel electrophoresis. The specific activity was up to 142.46 nmol C2H4/mg protein · min. It was homogeneous as shown by 1) a single band in the gel electrophorogram; 2) absence of Mo and tryptophan; 3) content of about 3.4 atoms of Fe per mole protein. The molecular weight of the Fe protein ofA. cylindrica was about 61

000 daltons as estimated by SDS-gel electrophoresis and calculated from the amino acid composition. The residues of aspartate and glutamate were about 2.6 times that of arginine and lysine in the Fe protein. Crossing Fe protein ofA. cylindrica with Mo−Fe protein ofAzotobacter vinelandii gave positive result. The reciprocal crossing also showed activity.

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references

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Eady, R.R., B.E. Smith, K.A. Cook et al., 1972. Nitrogenase ofKlebsiella pneumoniae. Purification and properties of the component proteins.Biochem. J. 128: 655–675.

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Weber, K. and M. Osborn, 1969. The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis.J. Biochem. 244: 4406

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