Physiological aspects of a high-co2 requiring mutant and the high-co2 growing cells of the cyanobacteriumSynechococcus pcc7942

Wu Tian-fu; Song Li-rong; Liu Yong-ding

doi:10.1007/BF02849091

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Physiological aspects of a high-co2 requiring mutant and the high-co2 growing cells of the cyanobacteriumSynechococcus pcc7942

OriginalPaper | Updated：2023-05-16

- Physiological aspects of a high-co2 requiring mutant and the high-co2 growing cells of the cyanobacteriumSynechococcus pcc7942
- Physiological aspects of a high-co2 requiring mutant and the high-co2 growing cells of the cyanobacteriumSynechococcus pcc7942
- 海洋湖沼学报（英文） 1998年16卷第1期页码：133-139
- Affiliations：
  
  Institute of Hydrobiology Chinese academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02849091
  中图分类号：
- 纸质出版：1998
- Accepted：
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Physiological aspects of a high-co2 requiring mutant and the high-co2 growing cells of the cyanobacteriumSynechococcus pcc7942[J]. 海洋湖沼学报（英文）, 1998,16(1):133-139.

Tian-fu, W., Li-rong, S. & Yong-ding, L. Physiological aspects of a high-co2 requiring mutant and the high-co2 growing cells of the cyanobacteriumSynechococcus pcc7942., Chin. J. Ocean. Limnol. 16, (Suppl 1), 133–139 (1998). https://doi.org/10.1007/BF02849091
Physiological aspects of a high-co2 requiring mutant and the high-co2 growing cells of the cyanobacteriumSynechococcus pcc7942[J]. 海洋湖沼学报（英文）, 1998,16(1):133-139. DOI： 10.1007/BF02849091.

Tian-fu, W., Li-rong, S. & Yong-ding, L. Physiological aspects of a high-co2 requiring mutant and the high-co2 growing cells of the cyanobacteriumSynechococcus pcc7942., Chin. J. Ocean. Limnol. 16, (Suppl 1), 133–139 (1998). https://doi.org/10.1007/BF02849091 DOI：

摘要

Abstract

A new chemically mutagenic mutant ofSynechococcus PCC7942 named high-CO2 requiring mutant

which could grow at 4% CO2 but could not grow at air levels of CO2

was isolated. Comparative studies on some physiological aspects of the mutant and high-CO2 growing cells (growing at 4% CO2) were conducted. The result showed that the mutant had lower growing rate

about 1/40th photosynthetic affinity to inorganic carbon

25% lower carbonic anhydrase (CA) activity

lower quenching rate of chlorophyll fluorescence

and about 1/2 alkalinization rate of the medium. The CA activity responses of the two types of cells to different concentration of CO2 were determined. Upon the addition

of inorganic carbon (Ci)

the rate of active Ci uptake described by the rate of chlorophyll fluorescence quenching of the mutant was obviously lower compared with that of the high-CO2 growing cells; the size of the internal inorganic carbon pool size detemined by the extent of fluorescence quenching of the mutant was also smaller.

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references

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