Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor

Liu Jian-guo; David O. Hall; Krishna K. Rao; Anatoly A. Tsygankov; Dmitry A. Sveshnikov

doi:10.1007/BF02849089

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Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor

OriginalPaper | Updated：2023-05-16

- Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor
- Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor
- 海洋湖沼学报（英文） 1998年16卷第1期页码：118-126
- Affiliations：
  
  1. Institute. of Oceanology. Chinese Academy of Sciences
  2. Division of Life Sciences King’s College London
  3. Institute of Soil Sciences and Photosynthesis Russian Acad. of Sciences Pushchino
  4. Department of Plant Physiology Biological Faculty Moscow State University
- Author bio：
- Funds：
  
  Contribution No.3297 from the Institute of Oceanology, Chinese Academy of Sciences.
- DOI：10.1007/BF02849089
  中图分类号：
- 纸质出版：1998
- Accepted：
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Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor[J]. 海洋湖沼学报（英文）, 1998,16(1):118-126.

Jian-guo, L., Hall, D.O., Rao, K.K. et al. Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor., Chin. J. Ocean. Limnol. 16, (Suppl 1), 118–126 (1998). https://doi.org/10.1007/BF02849089
Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor[J]. 海洋湖沼学报（英文）, 1998,16(1):118-126. DOI： 10.1007/BF02849089.

Jian-guo, L., Hall, D.O., Rao, K.K. et al. Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor., Chin. J. Ocean. Limnol. 16, (Suppl 1), 118–126 (1998). https://doi.org/10.1007/BF02849089 DOI：

摘要

Abstract

H2 photoproduction and nitrogenase activities in two strains ofAnabaena variabilis marked wild type ATCC 29413 and mutant PK84 exposed to thermal stress (temperature higher than the normal incubation temperature of 30°C) were studied. Cultures of both strains collected from any interval of logarithmic growth phase exhibited high H2 photoproduction and nitrogenase activities when exposed to limited time heat shock during the assay process. In contrast

the algal H2 photoproduction rate of both strains fluctuated with long term thermal stress caused by increasing the growth temperature from 30°C to 36°C. The changes of nitrogenase (the key H2 photobiosynthetic enzyme) activities in the mutant PK84 showed variation tendency similar to that of H2 photoproduction during exposure to thermal stress

indicating that fluctuation of H2 photoproduction in the mutant was mainly due to the variation of nitrogenase activities. A temporary maximal H2 photoproduction in the mutant PK84 (wild type ATCC29413) was observed when cells grew at 36°C for 14 (6) days. However

the responses of nitrogenase activities in the wild type to thermal stress were not completely similar to those in the mutant in spite of similar variations of H2 photoproduction in both strains. The data obtained in these studies suggested that the activities of other enzymes (in the wild strain) involved in H2 photoproduction were affected by thermal stress since H2 photoproduction maximized or dropped to 0 without variation tendency similar to that of nitrogenase activities. Furthermore

an enhancement of H2 photoproduction speed of the mutant strain cultured in a 4.4 L laboratory photobioreactor was also observed when it was subjected to short time continuous charge of argon

and temperature rise. All these results indicated that high temperature plays an important role in the photo-autotrophic H2 photoproduction

and that long term thermal stress is unfavourable for net H2 photoproduction in both strains ofA. variabilis though short-time heat shock is conducive to H2 photoproduction.

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references

Adams, M. W. W., Upchurch, R. G., Mortenson, L. E., 1980. An evaluation of N2 fixation and H2 production in fermentation culture.Annual reports on Fermentation Processes 4: 267–315.

Allen, B. M., Arnon D. I., 1955, Studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixationby Anabaena cylindrica Lemm.Plant Physiology 30: 366–372.

Blondin, P. A., Kirby, R. J., Bamum, S. R., 1993. The heat-shock response and acquired thermotolerance in 3 strains of cyanobacteria.Current Microbiology 26: 79–84.

Bothe, H., Kentemich, T., Heping, D., 1991. Recent aspects on hydrogenase-nitrogenase relationship in cyanobacteria.In: Polsinelli, M., Materassi, R., Vincenzini, M., (eds). Nitrogen fixation. Kluwer Academic Dordrecht, p.367–375.

Brass, S., Ernst, A., Boger, P., 1992. Induction and modification of nitrogense reductase in the unicellular cyanobacterium synechocystis BO-8402.Archives of Microbiology 158: 422–428.

Daday, A., Smith, G. D., 1983, The effect of nickel on the hydrogen metabolism inAnabaena azollae.FEMS Microbiology letter 20: 327–330.

Dilworth, M. J., 1966. Acetylene reduction by nitrogen-fixing preparations fromClostridium pasteurianum.Biochim. Biophys Acta.127: 285–294.

Fay, P., 1992. Oxygen relations of nitrogen fixation in cyanobacteria.Microbiol. Rev.56: 340–373.

Gallon, J. R., Pederson, D. M., Smith, D. J., 1993. The effect of temperature on the sensitivity of nitrogenase to o oxygen in the cyanobacteriaA. cylinerica (lemmermann) and gloeothece (nageli).New Phycologist 124: 251–257.

Lambert, G. R., Smith, G. D., 1981. Hydrogen uptake by the nitrogen-starved cyanobacteriumAnabaena cylindrica.Archives of Biochemistry and Biophysics 211: 360–367.

Lichtl R. R., Bazin, M. J., Hall, D. O., 1997. The biotechnology of hydrogen production byNostoc flagelliforme grown under chemostat conditions.Appl. Microbiol. Biotechnol, (in press).

Mackinny, G., 1941. Absorption of light by chlorohyll solutions.J. Biol. Chem. 140: 315–322.

Markov, S. A., Bazin, M. J., Hall, D. O., 1995. Hydrogen photoproduction and CO2 uptake by immobilizedAnabaena variabilis in hollow-fiber photobioreactor.Enzyme and Microbial Technology 17: 306–310.

Mikheeva, L. E., Schmitz, O., Shestakov, S. V. et al., 1995. Mutants of cyanobacteriumAnabaena variabilis altered in hydrogenase activities.Zeitschrift fur Naturforschung 50: 505–510.

Moreno, J., Rodriguez, H., Vargas, M. A. et al., 1995. Nitrogen-fixing cyanobacteria as source of phycobiliprotein pigments-composition and growth-performance of 10 filamentous Heterocystous strains.J. Applied Phycology 7: 17–23.

Panoff, J. M., 1993. control of a locus that is required for growth ofAnabaena Pcc7120 at low temperature.Current Microbiology 27: 273–276.

Sarma, T. A., Singh, D. P., 1994. Isolation and characterization of temperature-sensitive mutants ofAnabaena variabilis impaired in nitrogen-fixation.Folia Microbiologica 39: 296–300.

Smith, G. D., 1990. Hydrogen metabolism in cynobacteria.In: Kumar, H. D. (ed). Phycotalk, Vol 2. Rastogi and Co. Meerut. India. p. 131–143.

Sveshnikov, D. A., Sveshnikova, N. V., Rao, K. K. et al., 1997. Hydrogen metabolismof mutant forms ofAnabaena variabilis in continuous cultures and under nutritional stress.FEMS Microbiology Letter 147: 297–301.

Troshina, O. Y., Serbryakova, L. T., Lindblad, P., 1996. Induction of H2 uptake and nitrogenase activities in the cyanobacteriumAnabaena variabilis ATCC 29413. Effect of hydrogen and organic substrate.Current Microbiology 33: 11–15.

Tsygankov, A. A., Chan, V. N., Gogotov, I. N., 1991.Anabaena variabilis in continuous culture-growth and adaptation potential of its nitrogenase system.Microbiology 60: 591–595.

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