Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant

Shin-ichi Yano; Yoshikazu Kawata; Hiroyuki Kojima

doi:10.1007/BF02849087

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Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant

OriginalPaper | Updated：2023-05-16

- Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant
- Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant
- 海洋湖沼学报（英文） 1998年16卷第1期页码：105-108
- Affiliations：
  
  Agency of Industrial Science and Technology Osaka National Research Institute Ikeda
- Author bio：
- Funds：
- DOI：10.1007/BF02849087
  中图分类号：
- 纸质出版：1998
- Accepted：
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Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant[J]. 海洋湖沼学报（英文）, 1998,16(1):105-108.

Yano, Si., Kawata, Y. & Kojima, H. Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant., Chin. J. Ocean. Limnol. 16, (Suppl 1), 105–108 (1998). https://doi.org/10.1007/BF02849087
Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant[J]. 海洋湖沼学报（英文）, 1998,16(1):105-108. DOI： 10.1007/BF02849087.

Yano, Si., Kawata, Y. & Kojima, H. Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant., Chin. J. Ocean. Limnol. 16, (Suppl 1), 105–108 (1998). https://doi.org/10.1007/BF02849087 DOI：

摘要

Abstract

A unicellular cyanobacteriumSynechococcus sp. strain PCC 7002 was transformed with plasmid pQL1

on which β-lactamase gene (bla) and β-galactosidase gene (lacZ) were encoded. The transformant cells released β-lactamase into medium by an abrupt drop of osmotic pressure. This result indicates that this cyanobacterium recognizes and processes the signal sequence of β-lactamase

and accumulates the enzyme in periplasm. Repeated release of β-lactamase was possible by repeated osmotic shocks wihout

impairing cell viability. On the other hand

most of the β-galactosidase remained in cytoplasm under the osmotic shock.

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references

Brahamsha, B., 1996. A genetic manipulation system for oceanic cyanobacteria of the genusSynechococcus.Appl. Environ. Microbiol.62: 1747–1751.

Bush, K., Sykes, R. B., 1984. β-lactamase,In: Methods of enzymatic analysis, H. U. Bergmeyer, ed. Verlag Cheme, Weinheim, p. 280–285.

Kawata, Y., Yamano, N., Kojima, H., 1990. Development of host-vector systems for micro-algae I. Plasmid vector forSynechococcus sp.Bull. Gov. Ind. Res. Inst Osaka 41: 149–155.

Kawata, Y., Yamano, N., Kojima, H. et al., 1991. Expression of salmon growth hormone in the cyanobacteriumAgmenellum quadruplicatum.Biotech. Lett. 13: 851–856.

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