Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)

Kuang Mei; Wang Su-juan; Li Yao; Shen Da-leng; Zeng Cheng-kui

doi:10.1007/BF02849081

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Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)

OriginalPaper | Updated：2023-05-16

- Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)
- Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)
- 海洋湖沼学报（英文） 1998年16卷第1期页码：56-61
- Affiliations：
  
  1. Shanghai Fisheries University
  2. Fudan University
  3. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02849081
  中图分类号：
- 纸质出版：1998
- Accepted：
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Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)[J]. 海洋湖沼学报（英文）, 1998,16(1):56-61.

Mei, K., Su-juan, W., Yao, L. et al. Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)., Chin. J. Ocean. Limnol. 16, (Suppl 1), 56–61 (1998). https://doi.org/10.1007/BF02849081
Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)[J]. 海洋湖沼学报（英文）, 1998,16(1):56-61. DOI： 10.1007/BF02849081.

Mei, K., Su-juan, W., Yao, L. et al. Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)., Chin. J. Ocean. Limnol. 16, (Suppl 1), 56–61 (1998). https://doi.org/10.1007/BF02849081 DOI：

摘要

Abstract

Electroporation

PEC

PEG plus electroporation and Biolistics methods were tested in gene transformation ofP. yezoensis. The exogenousgus was from plasmid of pBI121 and pCAMBIA1301

both contain the CaMV35S promoter. The receptors included the protoplasts

tissues and free-living conchocelis filaments ofP. yezoensis. Several factors

for example

the voltage

capacitance and bivalent cations

etc.

were studied. Results show that these four methods are all efficient for gene transformation inP. yezoensis; and that PEG is the best one

with transformation efficiency of up to 4×10−5. GUS activity was detected 26 days after transformation by using PEG method.

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references

Cheney, D. P., Kurtzman, A. L., 1992. Progress in protoplast fusion and gene transfer in red algae, Abstracts, XIV International Seaweed Symposium, Brittany, France, No. 061.

Jefferson, R. A., Kavanagh T. A., Bevan, M. W., 1987. Assaying chimeric genes in plants: The GUS gene fusion system,Plant Mol. Biol. Report. 4:387–405.

Kuang, M., Wang S. J., Li Y. et al. 1998. Transient expression ofgus in four tissues of rhodophytes using biolistic particle delivery system.J. Fish. of China. 22(2):258–261. (in press, in Chinese)

Kulber, J. E., Minocha, S. C., Mathieson, A. C., 1994. Transient expression of the GUS reporter gene in protoplasts ofPorphyra miniata (Rhodophyta).J. Mar. Biotechnol.,1:165–169.

Qin, S., Zhang, J., Li, W. B. et al., 1994. Transient expression of GUS gene in Phaeophytes using biolistic particle delivery system.Oceanol. et Limnol. Sin. 25(4):353–356. (in Chinese, with English abstract)

Shen, D. I., Wu, M., 1995. Chromosomal and mutagenic study on the marine macroalga,Gracilaria tenuistipitata.J. Appl. Phycol. 7:25–31.

Shillito, R., Saul, M., Paszkowski, J. et al., 1985. High efficiency direct gene transfer to plants.Biotechnol.,3:1099.

Wang, S. J., Li, H., Li, Y. et al., 1994. Transient expression of GUS gene inPorphyra haitanensis by electroporation transformation.J. Shanghai Fish. Uni. 3(3):145–150. (in Chinese)

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