Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135

Yoshikazu Kawata; Shin-ichi Yano; Hiroyuki Kojima

doi:10.1007/BF02849075

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Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135

OriginalPaper | Updated：2023-05-16

- Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135
- Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135
- 海洋湖沼学报（英文） 1998年16卷第1期页码：17-24
- Affiliations：
  
  Agency of Industrial Science and Technology Osaka National Research Institute Ikeda
- Author bio：
- Funds：
- DOI：10.1007/BF02849075
  中图分类号：
- 纸质出版：1998
- Accepted：
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Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135[J]. 海洋湖沼学报（英文）, 1998,16(1):17-24.

Kawata, Y., Yano, Si. & Kojima, H. Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135., Chin. J. Ocean. Limnol. 16, (Suppl 1), 17–24 (1998). https://doi.org/10.1007/BF02849075
Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135[J]. 海洋湖沼学报（英文）, 1998,16(1):17-24. DOI： 10.1007/BF02849075.

Kawata, Y., Yano, Si. & Kojima, H. Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135., Chin. J. Ocean. Limnol. 16, (Suppl 1), 17–24 (1998). https://doi.org/10.1007/BF02849075 DOI：

摘要

Abstract

An efficient and simple method for constructing a genomic DNA library using a TA cloning vector is presented. It is based on the sonicative cleavage of genomic DNA and modification of fragment ends withTaq DNA polymerase

followed by ligation using a TA vector. This method was applied for cloning of the phytoene synthase genecrt B fromSpirulina platensis. This method is useful when genomic DNA cannot be efficiently digested with restriction enzymes

a problem often encountered during the construction of a genomic DNA library of cyanobacteria.

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references

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