Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines

Guo Hua-rong; Zhang Shi-cui; Li Hong-yan; Tong Shang-liang; Xiang Jian-hai

doi:10.1007/BF02848850

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Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines

OriginalPaper | Updated：2023-05-16

- Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines
- Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines
- 海洋湖沼学报（英文） 2002年20卷第3期页码：226-231
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Ocean University of Qingdao
- Author bio：
- Funds：
  
  This work was supported by China Ministry of Science and Technology (Contribution No. 819-04-06)
- DOI：10.1007/BF02848850
  中图分类号：
- 纸质出版：2002
- Accepted：
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Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines[J]. 海洋湖沼学报（英文）, 2002,20(3):226-231.

Hua-rong, G., Shi-cui, Z., Hong-yan, L. et al. Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines., Chin. J. Ocean. Limnol. 20, 226–231 (2002). https://doi.org/10.1007/BF02848850
Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines[J]. 海洋湖沼学报（英文）, 2002,20(3):226-231. DOI： 10.1007/BF02848850.

Hua-rong, G., Shi-cui, Z., Hong-yan, L. et al. Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines., Chin. J. Ocean. Limnol. 20, 226–231 (2002). https://doi.org/10.1007/BF02848850 DOI：

摘要

Abstract

Three continuous marine fish cell lines of FG (i.e.

Flounder Gill) from flounder (Paralichthys olivaceus) gill

SPH (i.e.

Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e.

Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin

were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However

the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG

SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However

morphological changes were found in SPH and RSBF compared to their original tissues. Therefore

the cellular morphology was still plastic in the relatively stable culture conditions

and it was possible that change of LDH patterns was related to morphological changes of fish cellsin vitro.

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references

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