Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody

Zhang Yong-an; Nie Pin

doi:10.1007/BF02847923

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Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody

OriginalPaper | Updated：2023-05-16

- Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody
- Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody
- 海洋湖沼学报（英文） 2002年20卷第4期页码：332-337
- Affiliations：
  
  State Key Laboratory of Freshwater Ecology and Biotechnology and Laboratory of Fish Diseases Institute of Hydrobiology Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Project No. 30025035 supported by NSFC, State Key Laboratory of Freshwater Ecology and Biotechnology (2000FB02), and the Chinese Academy of Sciences (KZ9511-B1-111).
- DOI：10.1007/BF02847923
  中图分类号：
- 纸质出版：2002
- Accepted：
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Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody[J]. 海洋湖沼学报（英文）, 2002,20(4):332-337.

Yong-an, Z., Pin, N. Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody., Chin. J. Ocean. Limnol. 20, 332–337 (2002). https://doi.org/10.1007/BF02847923
Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody[J]. 海洋湖沼学报（英文）, 2002,20(4):332-337. DOI： 10.1007/BF02847923.

Yong-an, Z., Pin, N. Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody., Chin. J. Ocean. Limnol. 20, 332–337 (2002). https://doi.org/10.1007/BF02847923 DOI：

摘要

Abstract

Serum immunoglobulin from the mandarin fish

or the so-called Chinese perch

Siniperca chuatsi (Basilewsky)

was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis gel

with molecular weights being estimated at 72 and 29 kDa

respectively. The tetrameric IgM ofS. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Western blot analysis. The antisera reacted strongly with the heavy chains ofS. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.

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references

Al-Harbi, A. H., Truax, R., Thune, R. L., 2000. Production and characterization of monoclonal antibodies against tilapiaOreochromis niloticus immunoglobulin.Aquaculture 188: 219–227.

Evelyn, T. P. T., 1996. Infection and disease.In: Iwama, G., Nakanishi, T., (eds.), The Fish Immune Systerm: Organism, Pathogen, and Environment. Academic Press, London, p. 339–366.

Gao, Q., Nie, P., Yao, W. J., 1999. Evidence of host blood feeding by the monogenean,Ancyrocephalus mogumdae (Monogenea: Ancyrocephalidae) from the gills of the mandarin fish,Siniperca chuatsi.Folia Parasitol. 46: 107–111.

Kattari, S. L., Piganelli, J. D., 1996. The specific immune system: Humoral defense.In: Iwama, G., Nakanishi, T., eds., The Fish Immune System: Organism, Pathogen, and Environment. Academic Press, London, p. 207–254.

Kobayashi, K., Hara, A., Takano, K. et al., 1982. Studies on subunit components of immunoglobulin M from a bony fish, the chum salmon (Oncorhynchus keta).Mol. Immunol. 19: 95–103.

Laemmli, U. K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227: 680–685.

Liang, Y. Q., Cui, X. Q., 1982. The eco-physiological characteristies of artificial propagation in mandarin fish (Siniperca chuatsi).Acta Hydrobiol. Sin.,16: 90–92. (in Chinese with English abstract).

Liu, J., Cui, Y., Liu, J., 1998. Food consumption and growth of two piscivorous fishes, the mandarin fish and the Chinese snakehead.J. Fish Biol. 53: 1071–1083.

Lobb, C. J., Olson, M. O., Clem, L. W., 1984. Immunoglobulin light chain classes, in a teleost fish.J. Immunol. 132: 1917–1932.

Lobb, C. J., Olson, M. O., 1988. Immunoglobulin heavy chain isotypes in a teleost fish.J. Immunol. 141: 1236–1245.

Magnadottir, B., 1998. Comparison of immunoglobulin (IgM) from four fish species.Buvisindi 12: 47–59.

Nishida, H., Enokida, T., Hiramatsu, N. et al., 1998. Purification of immunoglobulin M (IgM) in serum of Japanese flounder (Paralichthys olivaceus).Bull. Facul. Fish. Hokkaido Univ. 49: 157–164.

Nie, P., 1996. Co-occurrence and microhabitat ofAncyrocephalus mogumdae (Monogenea) andHemeguya weishanensis (Myxosporea) on gills of the mandarin fish,Siniperca chuatsi.Folia Parasitol. 43: 272–276.

Nie, P., Guo, Q. L., Yao, W. J., 1996. The nutrition of a monogenean,Ancyrocephalus mogumdae Gussev parasitic on gills of the mandarin fish,Siniperca chuatsi (Basilewsky).Acta Hydrobiol. Sin. 20: 297–301. (in Chinese with English abstract)

Philips, J. O., Ourth, D. D., 1986. Isolation and molecular weight determination of two immunoglobulin heavy chains in the channel carfish,Ictalurus punctatus.Comp. Biochem. Physiol. 85B: 49–54.

Pilström, L., Bengtén, E., 1996. Immunoglobulin in fish? genes, expression and structure.Fish Shellfish Immunol. 6: 243–262.

Sanchez, C., Dominguez, J., Coll, J., 1989. Immunoglobulin heterogeneity in the rainbow trout,Salno gairdneri Richardson,J. Fish Dis. 12: 459–465.

Scapigliati, N., Romano, N., Abelli, L., 1999. Monoclonal antibodies in fish immunology: identification, ontogeny and activity of T- and B-lymphocytes.Aquaculture 172: 3–28.

Smith, S. A., Gebhard, D. H., Housman, J. M. et al., 1993. Isolation, purification and molecular-weight determination of serum immunoglobulin fromOreochromis aureus.J. Aquat. Anim. Health 5: 23–25.

Wilson, M. R., Warr, G. W., 1992. Fish immunoglobulins and the genes that encode them.Ann. Rev. Fish Dis. 2: 201–221.

Wu, X. W., Rao, J. Z., He, B. W., 1992. The history of the Chinese freshwater fisheries.In: Liu, J. K., He, B. W., (eds.) Cultivation of the Chinese Freshwater Fishes. Science Press, Beijing, p. 5–29.

Yang, G. W., An, L. G., Wang, C. F. et al., 1999. Comparative studies on the immunoglobulin in intestine mucus and serum ofCyprinus carpio.J. Fish. Sci. China 6: 109–110 (in Chinese)

Zhang, G. H., Cao, W. X., Chen, Y. Y., 1997. Effects of fish stocking on lake ecosystems in China.Acta Hydrobiol. Sin. 21: 271–280. (in Chinese with English abstract)

Zhang, Y. A., Nie, P., 1998. Isolation, purification and molecular weight determination of serum immunoglobulin from the mandarin fish,Siniperca chuatsi.Acta Hydrobiol. Sin. 22: 192–194. (in Chinese)

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