Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF

Guo Hua-rong; Zhang Shi-cui

doi:10.1007/BF02847922

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Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF

OriginalPaper | Updated：2023-05-16

- Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF
- Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF
- 海洋湖沼学报（英文） 2002年20卷第4期页码：323-331
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. College of Marine Life Sciences Ocean University of Qingdao
- Author bio：
- Funds：
  
  This work was supported by Ministry of Sciences and Technology of China (G1999012006).
- DOI：10.1007/BF02847922
  中图分类号：
- 纸质出版：2002
- Accepted：
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Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF[J]. 海洋湖沼学报（英文）, 2002,20(4):323-331.

Hua-rong, G., Shi-cui, Z. Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF., Chin. J. Ocean. Limnol. 20, 323–331 (2002). https://doi.org/10.1007/BF02847922
Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF[J]. 海洋湖沼学报（英文）, 2002,20(4):323-331. DOI： 10.1007/BF02847922.

Hua-rong, G., Shi-cui, Z. Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF., Chin. J. Ocean. Limnol. 20, 323–331 (2002). https://doi.org/10.1007/BF02847922 DOI：

摘要

Abstract

A continuous marine fish cell line RSBF (i. c. Red Sea Bream Fin) was utilized to screen the cytotoxicity and genotoxicity of polyethylenimine (PEI) and nickel chloride (NiCl2) in this study on the deleterious effects of aquatic genotoxins on fish. At the 0.01 to 1 μg/ml concentration tested

PEI had acute toxicity to the treated RSBF cells (IC50=1.12

0.92

0.88 and 0.64 μg/ml PEI for time 0 h

24 h

48 h and 72 h after treatment

respectively) and markedly inhibited their proliferation in a dose-dependent manner. At the 0.001 to 5 μmol/L concentration tested

NiCl2 posed no acute toxicity but significantly stimulated their growth (107%–214% of control). Random amplified polymorphic DNA (RAPD) technique was used to detect the genotoxic effects of PEI and NiCl2 by comparing the RAPD banding patterns of the control and treated cells. RAPD analysis indicated that at the concentrations tested

PEI was more genotoxic than NiCl2 to RSBF cells; that there was a slight dose-dependent response in the genotoxic effect of PEI but not NiCl2; and that RAPD technique might provide a sensitive

non-specific genotoxic endpoint. And the potent cytotoxicity and genotoxicity of PEI on fish cells showed that we should be cautious in utilizing it as gene

vector in fish gene transfer and human gene therapy.

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references

Babich, H., Shopsis, C., Borenfreund, E., 1986. In vitro cytotoxicity testing of, aquatic pollutants (cadmium, copper, zinc, nickel) using established fish cell lines.Ecotoricology and Environmental Safety 11: 91–99.

Becerril, C., Ferrero, M., Sanz, F. et al., 1999. Detection of mitomycin C-induced genetic damage in fish cell by use of RAPD.Mutagenesis 14 (5): 449–456.

Bikales, M., Overberger, M., 1985. Encyclopedia of Polymer Science and Engineering. Wiley, New York. Vol. 1.

Boussif, O., Lezoualc'h, F., Zanta, M. A. et al., 1995. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine.Proc. Natl. Acad. Sci. USA 92: 7297–7301.

Ciccarelli, R. B., Wetterhahn, K. E., 1984. Nickel-bound chromatin, nucleic acids, and nuclear proteins from kidney and liver of rats treated with nickel carbonate in vivo.Cancer Research 44: 3892–3897.

Costa, M., Abbracchio, M. P. Simmons-Hansen, J., 1981. Factors influencing the phagocytosis, neoplastic transformation, and cytotoxicity of particulate nickel compounds in tissue culture systems.Toxicity and Applied Pharmacology 60: 313–323.

Costa, M., Heck, J. D., Robison, S. H., 1982. Selective phagocytosis of crystalline metal sulfide particles and DNA strands breaks as a mechanism for the induction of cellular transformation.Cancer Research 42: 2757–2763.

Ericson, G., Liewenborg, B., Lindesjöö, E., et al., 1999. DNA adducts in perch (Perca fluviatilis) from kidney and liver of rats treated with nickel carbonate in vivo.Cancer Research 44: 3892–3897.

Fletcher, G. G., Rossetto, F. E., Turnbull, J. D. et al., 1994. Toxicity, uptake and mutagenicity of particulate and soluble nickel compounds.Environ. Health Perspect 102 (Suppl. 3): 69–79.

Gardner, G. G., Yevich, P. P., Harsbarger, J. C. et al., 1991. Carcinogenicity of Black Rock Harbor sediment to the eastern oyster and trophic transfer of Black Rock Harbor carcinogens from the blue mussel to the winter flounder. Environ.Health Perspect 90: 53–66.

GESAMP (IMO/FAO/Unesco/WMO/WHO/IAEA/UN/UNEP Joint Group of Experts on the Scientific Aspects of Marine Pollution), 1991. Review of potentially harmful substances? carcinogens. Reports and Studies No. 46 World Health Organization, Geneva.

Grandjean, P., Andersen, O., Nielsen, G. D., 1988. Carcinogenicity of occupational nickel exposure: an evaluation of the epidemiological evidence.Am. J. Med. 13: 193–209.

Huang, X., Frenkel, K., Klein, C. B. et al., 1993. Nickel induces increase on oxidants in intact cultured mammalian cells as detected by dichlorofluorescein fluorescence.Toxicol. Appl. Pharmacol. 120: 29–36.

ICNCM, 1990. Report of international committee on nickel carcinogenesis in man, Scandinavian Journal of Work, Environment and Health, Vol. 16, Helsinki, Finland, pp. 1–82.

Kirsch-Volders, M., Aarderna, M., Elhajouji, A., 2000. Concepts of threshold in mutagenesis and carcinogenesis.Mutation Research 464: 3–11.

Kocan, R. M., Landolt, M. L., Sabo, K. M., 1979. In vitro toxicity of eight mutagens/carcinogens for three fish cell lines.Bull. Environm. Contam. Toxicol. 23: 269–274.

Mitchelmore, C. L., Chipman, J. K., 1998. Detection of DNA strand breaks in brown trout (Salmo trutta) hepatocytes and blood cells using the single cell gel electrophoresis (comet) assay.Aquatic Toxicity 41: 161–182.

Mix, M., 1986. Cancerous disease in aquatic animals and their association with environmental pollutants: a critical literature review.Mar. Environ. Res. 20: 1–141.

Nei, M., Li, W. H., 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases.Proc. Natl. Acad. Sci. USA 76: 5269–5273.

Patierno, S. R., Dirscherl, L. A., Xu, J. 1993. Transformation of rat tracheal epithelial cells to immortal growth variants by particulate and soluble nickel compounds.Mutation Research 300: 179–193.

Sambrook, J., Fritsch, E. F., Maniatis, T., 1989. Molecular cloning: a laboratory mannal. 2nd ed. Cold Spring Harbor Laboratory Press, USA.

Sanchez, P., Llorente, M. T., Castano, A., 2000. Flow cytometric detection of micronuclei and cell cycle alterations in fish-derived cells after exposure to three model genotoxic agents: mitomycin C, vincristine sulfate and benzo(a)pyrene.Mutation Research 465(1–2): 113–122.

Sunderman, F. J., Maenza, R. M., 1976. Comparisons of carcinogenicity of nickel compounds in rats.Res. Commun. Chem. Pathol. Pharmacol. 14: 319–332.

Swenberg, J. A., Han, A., Koc, H. et al., 2000. DNA adducts: effects of low exposure to ethylene oxide, vinyl chloride and butadiene.Mutation Research 464: 77–86.

Tong, S. L., Miao, H. Z., Li, H., 1998. Three new continuous fish cell lines of SPH, SPS and RSBF derived from sea perch (Lateolabrax japonicus) and red sea bream (Pagrosomus major).Aquaculture 169: 143–151.

Welsh, J., McClelland, M., 1990. Fingerprinting genomes using PCR with arbitrary primers.Nucleic Acid Research 18(24): 7213–7218.

Williams, J. G. K., Kubelik, A. R., Livak, K. J. et al., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.Nucleic Acid Research 18(22): 6531–6535.

Würgler, F. E., Kramers, P. G. N., 1992. Discussion forum: environmental effects of genotoxins (ecogenotoxicology).Mutagenesis 7(5): 321–327.

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