The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage

Zhang Yujiang; Zhan Songmei; Cao Pengli; Liu Ning; Chen Xuehong; Wang Yuejun; Wang Chunbo

doi:10.1007/BF02847161

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The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage

OriginalPaper | Updated：2023-05-16

- The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage
- The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage
- 海洋湖沼学报（英文） 2005年23卷第3期页码：357-362
- Affiliations：
  
  1. Medical College Qingdao University Qingdao
  2. Yellow Sea Fishery Research Institute Qingdao
- Author bio：
- Funds：
  
  This work was supported by the National Natural Science Foundation of China (No. 39970638) and the Science and Technology Bureau of Qingdao (No. 2001-28-50).
- DOI：10.1007/BF02847161
  中图分类号：
- 纸质出版：2005
- Accepted：
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The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage[J]. 海洋湖沼学报（英文）, 2005,23(3):357-362.

Yujiang, Z., Songmei, Z., Pengli, C. et al. The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage., Chin. J. Ocean. Limnol. 23, 357–362 (2005). https://doi.org/10.1007/BF02847161
The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage[J]. 海洋湖沼学报（英文）, 2005,23(3):357-362. DOI： 10.1007/BF02847161.

Yujiang, Z., Songmei, Z., Pengli, C. et al. The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage., Chin. J. Ocean. Limnol. 23, 357–362 (2005). https://doi.org/10.1007/BF02847161 DOI：

摘要

Abstract

To investigate the effect of polypeptide fromChlamys farreri (PCF) on NHDFin vitro

we modeled oxidative damage on normal human dermal fibroblasts (NHDF) exposed to ultraviolet B (UVB). In this study

3-[4

5-Dimethylthiazol-2-yl]-2

5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) were tested to measure cell viability. Enzymes including superoxide dismutase (SOD)

glutathione peroxidase (GSH-PX)

catalase (CAT) and xanthine oxidase (XOD) were determined biochemically. Total antioxidative capacity (T-AOC) and anti-superoxide anion capacity (A-SAC) were also determined. Ultrastructure of fibroblasts was observed under transmission electron microscope. The results showed that: UVB (1.176×10−4 J/cm2) suppressed the growth of fibroblasts and the introduction of PCF (0.25%–1%) before UVB reduced the suppression in a concentration-dependent manner. PCF could enhance the activities of SOD

GSH-PX and T-AOC as well as A-SAC. Also PCF could inhibit XOD activity

while it did not affect CAT activity. Ultrastructure of fibroblasts were damaged after UVB irradiation

concentration-dependent PCF reduced the destructive effect of UVB on cells. These results indicated that PCF can protect human dermal fibroblasts from being harmed by UVB irradiation via its antioxidant proerty.

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references

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