Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis

Liu Hui-lian; Shuai Li; Duan De-lin; Xu Huai-shu

doi:10.1007/BF02846612

Your Location：

Home >

Browse articles >

Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis

OriginalPaper | Updated：2023-05-16

- Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis
- Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis
- 海洋湖沼学报（英文） 2002年20卷第1期页码：62-66
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Department of Environmental Science Qingdao University
  3. Department of Marine Biology Ocean University of Qingdao
- Author bio：
- Funds：
  
  Contribution No. 2381 from the Institute of Oceanology, Chinese Academy of Sciences.;This research was subsidized by Special Funds for Major State Basie Research G1999012004 and the Foundation of the Chinese Academy of Sciences KN95-03-04.
- DOI：10.1007/BF02846612
  中图分类号：
- 纸质出版：2002
- Accepted：
Scan QR Code
Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis[J]. 海洋湖沼学报（英文）, 2002,20(1):62-66.

Hui-lian, L., Li, S., De-lin, D. et al. Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis, ., Chin. J. Ocean. Limnol. 20, 62–66 (2002). https://doi.org/10.1007/BF02846612
Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis[J]. 海洋湖沼学报（英文）, 2002,20(1):62-66. DOI： 10.1007/BF02846612.

Hui-lian, L., Li, S., De-lin, D. et al. Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis, ., Chin. J. Ocean. Limnol. 20, 62–66 (2002). https://doi.org/10.1007/BF02846612 DOI：

摘要

Abstract

After discarding marine microorganisms from conchocelis ofPorphyra yezoensis andPorphyra haitanensis

their axenic cultures were obtained through treatment with antibiotics. Antibiotic disc tests were carried out to determine the effectiveness of each antibiotic in eliminating contaminating microorganisms. Five of 12 antibiotics tested were selected and used to produce the axenic cultures in this study

which showed that 200 μg/mL streptomycin

250 μg/mL penicillin

252 μg/mL kanamycin

30 μg/mL chloramphenicol were effective concentrations for eliminating microorganisms from conchocelis when antibiotics were added singly step by step; whereas simultaneous combination of 150 μg/mL streptomycin

250 (or 350) μg/mL penicillin

150 (or 250) μg/mL kanamycin

70 μg/mL neomycin and 200 μg/mL chloramphenicol was also effective for producing the axenic cultures. However

it seemed that the treatments with antibiotics applied individually were more feasible than those will all antibiotics added at the same time. This may be due to the combined inhibiting effect of antibiotics on the growth and development of conchocelis.

关键词

Keywords

references

Aguirre-Lipperheide, M., Estrada-Rodríguez, F. J., Evans, L. V., 1995. Facts, problems, and needs in seaweed tissue culture: an appraisal.J. Phycol. 31: 677–688.

Ambler, C. D., Reed, D. C., Neushul, M., 1992. The microclimate inhabited by macroalgal propagules.Br. Phycol. J. 27: 253–270.

Boller, T., 1995. Chemoperception of microbial signal in plant cells.Ann. Rev. Plant Physiol. & Plant Mol. Biol. 46: 189–214.

Chen, L. C. M., McCracken, I., 1993. An antibiotic protocol for preparing axenic cell cultures ofPorphyra linearis.Bot. Mar. 36: 29–33.

Cottrell, M. T., Suttle, C. A., 1993. Production of axenic cultures ofMicromonas pusilla (Prasinophyceae) using antibiotics.J. Phycol. 29: 385–387.

Duan, D. L., Xu, L., Fei, X. et al., 1995. Marine organisms attached on seaweed surfaces in Jiaozhou Bay, China.Wor. J. Microbiol. & Biotech. 11: 351–352.

Evans, L. V., Trewavas, A. J., 1991. Is algal development controlled by plant growth substances?J. Phycol. 27: 322–326.

Hunt, B. E., Mandoli, D. F., 1992. Axenic cultures ofAcetabularia (Chlorophyta): a decontamination protocol with potential application to other algae.J. Phycol. 28: 407–414.

Ji, W. S., Xu, B., Xu, H. S. et al., 1991. Studies on the attachment of marine bacteria to biotic and abiotic surfaces.J. Ocean. Univ. Qingdao. 21(2): 61–68. (in Chinese with English abstract)

Kishor, P. B., Mehta, A. R., 1988. Effect of rifamycin and gentamicin on growth, organogensis and arbohydrate metabolising enzymes in callus cultures of tobacco.Phytomorphology 38 (4): 327–331.

Kooistra, W. H. C. F., Boele-Bos, S. A., Stam, W. T., 1991. A method for obtaining axenic algal cultures using the antibiotic cefotaxime with emphasis onCladophoropsis membrancea (Chlorophyta).J. Phycol. 27: 656–658.

McCracken, I. R., 1989. Purifying algal cultures- a review of chemical methods.Proc. N. S. Inst. Sci. 38: 145–168.

Provasoli, L., 1968. Media and prospects for the cultivation of marine algae.In: A. Watanabe & A. Hattori eds, Cultures and Collections of Algae. Proc. U.S.-Japan Conf. Hakone, Sept. 1966. Jap. Soc. Plant Physiol. p. 63–75.

Tait, M. I., 1991. A case for non-axenic algal cultures. In: Garcia-Reina, G., Pedersen, M. eds, Proc. COST48, Seaweed cellular biotechnology, physiology and intensive cultivation., Las Palmas, Canary Islands, Spain, p. 41–45.

Tatewaki, M., Provasoli, L. P., Pinnter, I. J., 1983. Morphogenesis ofMonostroma oxyspermum (Kutz.) doty (Chlorophyceae) in axenic culture, especially in bialgal culture.J. Phycol. 19: 409–416.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effects of oxytetracycline dihydrate and sulfamethoxazole on Microcystis aeruginosa and Chlamydomonas microsphaera

Studies on color type variants from mutagenized protoplasts ofPorphyra haitanensis Chang et Zheng &P. Yezoensis ueda (rhodophycease)

Explant exenisation for tissue culture in marine macroalgae

Studies on intercropping ofPorphyra haitanensis T. J. Chang et B. F. Zheng with Bay ScallopArgopecten irradians Lamarck

Effect of cytokinins and auxins on the growth of free-living conchocelis ofPorphyra yezoensis