Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio

M.S. Sawant; Zhang Shicui; Wang Qingyin

doi:10.1007/BF02845144

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Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio

OriginalPaper | Updated：2023-05-16

- Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio
- Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio
- 海洋湖沼学报（英文） 2005年23卷第1期页码：54-59
- Affiliations：
  
  1. College of Marine Life Sciences Ocean University of China
  2. Yellow Sea Fisheries Research Institute
- Author bio：
- Funds：
  
  This work was supported by Ministry of Science and Technology (MOST) of China (G 1999012005).
- DOI：10.1007/BF02845144
  中图分类号：
- 纸质出版：2005
- Accepted：
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Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio[J]. 海洋湖沼学报（英文）, 2005,23(1):54-59.

Sawant, M., Shicui, Z. & Qingyin, W. Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio, ., Chin. J. Ocean. Limnol. 23, 54–59 (2005). https://doi.org/10.1007/BF02845144
Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio[J]. 海洋湖沼学报（英文）, 2005,23(1):54-59. DOI： 10.1007/BF02845144.

Sawant, M., Shicui, Z. & Qingyin, W. Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio, ., Chin. J. Ocean. Limnol. 23, 54–59 (2005). https://doi.org/10.1007/BF02845144 DOI：

摘要

Abstract

Zebrafish (Danio rerio) genetic screens allow isolation of a wide array of problems in vertebrate biology. The effects of base analogues 5-bromouracil and 6-aminopurine on the development of zebrafish embryos are reported for the first time in this study. The early development of the zebrafish embryos was little affected by 5-bromouracil and 6-aminopurine

while the late development (organogenesis) was significantly impaired. Embryos exposed to 5-bromouracil mainly showed curled tail

wavy body

golden pigmentation and the mouth with protruding lower jaw. 6-aminopurine-treated embryos had defective anterior structures

curled tails and wavy body. RAPD analysis showed that the majority of 5-bromouracil- and 6-aminopurine-treated larvae and fish shared banding patterns in common with the control

suggesting that most mutagenesis induced by these agents are point mutations. However

some fish derived from 5-bromouracil-treated embryos had golden (gol) pigmentation; and RAPD analysis revealed that their band patterns differed from those of the control. Possibly

5-bromouracil can occasionally cause relatively extensive changes in the fish genome. The results of this study may provide valuable help for detailed studies of mutagenesis.

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references

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