Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans

Wu Xiaonan; Zhou Baicheng; C. K. Tseng

doi:10.1007/BF02844744

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Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans

OriginalPaper | Updated：2023-05-16

- Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans
- Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans
- 海洋湖沼学报（英文） 1992年10卷第2期页码：135-144
- Affiliations：
  
  .M.B.L. Institute of Oceanology,E
- Author bio：
- Funds：
  
  Contribution No 1717 from the Institute of Oceanology, Academia Sinica, This subject was supported by the National Science Foundation of China.
- DOI：10.1007/BF02844744
  中图分类号：
- 纸质出版：1992
- Accepted：
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Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans[J]. 海洋湖沼学报（英文）, 1992,10(2):135-144.

Xiaonan, W., Baicheng, Z. & Tseng, C.K. Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans, ., Chin. J. Ocean. Limnol. 10, 135–144 (1992). https://doi.org/10.1007/BF02844744
Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans[J]. 海洋湖沼学报（英文）, 1992,10(2):135-144. DOI： 10.1007/BF02844744.

Xiaonan, W., Baicheng, Z. & Tseng, C.K. Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans, ., Chin. J. Ocean. Limnol. 10, 135–144 (1992). https://doi.org/10.1007/BF02844744 DOI：

摘要

Abstract

Polyc·lonal antibodies raised against LHC II isolated from SDS-solubilizedBryopsis corticulans thylakiod membranes by SDS-PAGE

were characterised by double immunodiffusion

Rocket immunoelectrophoresis and antigen-antibody crossed immunoelectro-phoresis assays showed the antibodies had strong cross-reaction with allB. corticulans LHC II components (even with those which were incubated in boiling water) and showed immunological cross-reactivity with LHC II polypeptides of spinach and the marine green algaCodium fragile. The results suggested that LHC II of different species had similar antigenic determinants and also conservation of amino acid sequences of the polypeptides during evolution

and that the antibodies could cross react with apoproteins of D2 proteins (which contain P680) fromB. corticulans

spinach andC. fragile

but not with apoproteins of P700 Chl-proteins. Our results indicated some similarities in primary structure between LHC II of different species

and between LHC II and D2 proteins of marine green algae and spinach. Our finding that D2 and P700 Chl-proteins are not immunologically related suggested that P700 Chl-proteins and D2 proteins pass through independent evolutionary pathways.

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references

Anderson, J. M., 1980. P700 content and polypeptide profile of chlorophyll-protein complexes of spinach and barley thylakoids.Biochim. Biophys. Acta 591: 113–126.

Anderson, J. M., P. K. Goodchild & D. J. Evance, 1987. Immunological cross-reactivity between the light-harvesting chlorophylla/b-protein of a marine green alga and spinach.Physiol. Plant 70: 597–602.

Apel, K., 1977. The light-harvesting chlorophylla/b-protein complex of the green algaAcetebularia mediterranea.Biochim. Biophys. Acta 462: 390–402.

Arnon, D. I., 1949. Copper enzymes in isolated chloroplast polyphenol oxidase inBeta vulgaris.Plant Physiol. 24: 1–15.

Chua, N. H. & F. Blomberg, 1979. Immunochemical studies of thylakoid membrane polypeptides from spinach andChlamydomonas reinhardtii.J. Biol. Chem. 254: 215–223.

Evans, P. K. & J. M. Anderson, 1986. The chlorophylla/b-proteins of PS I and PS II are immunologically related.FEBS Lett.,199: 227–233.

Thaler, T. & F. A. Jay, 1985. Monoclonal antibodies specific for the light-harvesting chlorophylla/b-protein complex (LHC).FEBS Lett 188: 21–26.

Vierling, E. & R. S. Alberte, 1983. P700 chlorophylla-protein.Plant Physiol. 72: 625–633.

Wu, X. N., B. C. Zhou & C. K. Tseng, 1991. Immunological cross-reactions between P700 chlorophyll-proteins isolated from two marine green algae and one higher plant.Chin. J. Oceanol. Limnol. 9: 97–105.

Xie, N. W., (ed) 1979. Experimental Approach of Immunology. Science Press, Beijing, pp. 15–20. (in Chinese)

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