Explant exenisation for tissue culture in marine macroalgae[J]. 海洋湖沼学报(英文), 1992,10(3):268-275.
Xuewu, L., Kloareg, B. Explant exenisation for tissue culture in marine macroalgae., Chin. J. Ocean. Limnol. 10, 268–275 (1992). https://doi.org/10.1007/BF02843827
Explant exenisation for tissue culture in marine macroalgae[J]. 海洋湖沼学报(英文), 1992,10(3):268-275. DOI: 10.1007/BF02843827.
Xuewu, L., Kloareg, B. Explant exenisation for tissue culture in marine macroalgae., Chin. J. Ocean. Limnol. 10, 268–275 (1992). https://doi.org/10.1007/BF02843827DOI:
Explant exenisation for tissue culture in marine macroalgae
摘要
Abstract
Unialgal explants fromLaminaria digitata
and from a variety of red algae
were obtained by hand removing the visible epiphytes
and stirring the tissue in the presence of glass beads. Two antibiotic mixtures were found to be efficient in removing the contaminating fungi and bacteria from the algae. The procedure proved suitable as a primary step in the tissue culture of the investigated species.
关键词
Keywords
references
Ballantine, D. L., 1979. The distribution of algal epiphytes on macrophyte hosts offshore from La Parguera, Puerto Rico.Bot. Mar. 22: 107–111.
Berland, B. R. and S. Y., Maestrini, 1969. Study of the bacteria associated with marine algae in culture. II: Action of antibiotic substance.Mar. Bio. 3: 334–335.
Berland, B. R., D. J. Bonin, and S. Y., Maestrini, 1972. Are some bacteria toxic for marine algae?Mar. Bio. 12: 189–193.
Bradley, P. M., D. P. Cheney, and N., Sega, 1988. One step antibiotic disk method for obtaining axenic culture of multicellular marine algae.Plant Cell, Tissue and Organic Culture 12: 55–60.
Brown, L., 1982. Production of axenic cultures of algae by an osmotic method.Phycologia.21: 408–410.
Chen, L. C. M., 1982. Callus-like formation fromIrish moss.Biol. Bull. nat. Taiwan Normal Univ. 17: 63–67.
Cheney, D. P., A. H. Luistro, and M. Bradley, 1987. Carrageenan analysis of tissue cultures and whole plants ofAgardhiella subulata.Proc. Int. Seaweed Symp. 12: 161–166.
Darveau, R. P. and D. L. Lynch, 1977. The antibiotic activity of prodigiosins against certain blue-green and green algae.Phycologia.16(3): 349–351.
Fries, L., 1963. On cultivation of axenic red algae.Physiol. Plant. 16: 695–708.
Fries, L., 1970. The influence of microamounts of organic substances other than vitamins on growth of some red algae in axenic culture.Br. Phycol. J. 5: 39–46.
Fries, L., 1984. Induction of plantlets in axenically cultivated rhizoids ofFucus spiralis.Can. J. Bot. 62: 1616–1622.
Fries, L., 1985. Propagation ofFucus (Fucales, Phacophyta) by hairs with trichothallic growth.Phycologia.24: 481–484.
Gibor, A., M., Polne, M. Biniaminov, and M., Neushul, 1980. Exploratory studies of vegetative propagation of marine algae: procedure for obtaining axenic tissue.Proc. Int. Seaweed Sym. 10: 587–593.
Guillard, R. R. L. and J. H. Ryther, 1962. Studies on marine planktonic diatoms I.Cyclotella nana Hustedt andDetonula confervacea (Cleve)Gran.Can J Microbiol. 8: 229–239.
Gusev, M. V., A. H., Tambiev, N. N. Kirikova,et al, 1984. Obtaining of axenic explants from marine algae thallus.Izv. Acad. Nauk USSR ser. Bol. 5: 761–721. (in Russian)
Gusev, M. V., A. H. Tambiev, N. N. Kirikova,et al., 1987. Callus formation in seven species of agarophyte marine algae.Mar. Biol. 95: 593–597.
Hopkin, R. and J. M. Kain, 1978. The effects of some pollutants on the survival growth and respiration ofLaminaria hyperborea.Est. Coast. Mar. Sci. 7: 531–553.
Kavi Kishor, P. B. and A. R. Mehta, 1988. Effect of Rifampicin and Gentamycin on growth, organogenesis and carbohydrate metabolising enzymes in callus cultures ofTobacco.Phytomorphology.38 (4) 327–331.
Lawlor, H. J., J. A. McComb, and M. A. Borowitzka, 1988. The Development of Filamentous and Callus-like Growth in Axemic Tissue Culture ofEcklonia radiata (Phaeophyta).In: Algal Biotechnology. Staleret al. Eds., Elsevier Appl Sciences, pp. 139–150.
Lee, T. F., 1985. Aposporous gametophytes formation in stipe explants fromLaminaria saccharina (Phaeophy ta).Bot. Mar. 28: 179–185.
Lewin, J., 1966. Studies metabolism in diatoms V. Germanium dioxide, a specific inhibitor of diatom growth,Phycologia 6: 1–12.
Liu, X. W., 1987. Tissue, Cell and Callus Culture ofPterocladia andPorphyra Seaweeds. MSc Thesis, Victoria Univ. of Wellington, New Zealand, 86 pp.
Liu, X. W. and M. E. Gordon, 1987. Tissue and cell culture of New ZealandPterocladia andPorphyra species.Proc. Int. Seaweed Symp. 12: 147–154.
Markham, J. W. and E. Hagmeier, 1982. Observation on the effects of germanium dioxide on the growth of macro-algae and diatoms.Phycol. 21(2): 125–130.
McKracken, I. R., 1989. Purifying algal cultures. A review of chemical methods.Proc. N. S. Inst. Sci. 38: 145–168.
Polne-Fuller, M. and A. Gibor, 1987. Calluses and callus-like growth in seaweeds: induction and culture.Proc. Int. Seaweed Symp,12: 131–138.
Polne, M., A. Gibor, and M. Neushul, 1980. The use of ultrasound for the removal of marine-algal epiphytes.Bot. Mar. 23: 731–734.
Printer, I. and L. Provasoli, 1968. Heterotrophy in subdued light of 3 chrysochromulina species.Bull. Misoki Mar. Bio. Inst., Kyoto Univ. 12: 25–31.
Provasoli, L., 1968. Media and Prospects for the Cultivation of Marine Algae.Proc. Jpn. Conf. Hacone. pp. 63–75.
Saga, N. and Y. Sakai, 1982. A new method for pure culture of macrosćopic algae, the one step selection method.Jap. J. Phycol. 30: 40–43.
Santos, I. and R. Salema, 1989. Penicillins and activity of nitrogen metabolism enzymes in plant tissue cul-Ture.Plant Science,59: 119–125.
Shephard, D. C. 1970. Axenic Culture of Acetabularia in a Synthetic Medium. In: Methods in Cell Physiology. Ed. Prescott, D. M.IV: pp. 49–69.
Yan, Z. M., 1984. Studies on tissue culture ofLaminaria japonica andUndaria pinnatifida., Hydrobiologia.116/117: 314–316.
Zhang, D., 1983. Study on the protoplast preparation, culture and fusion of somatic cells from two species of Green algae—Ulva linza andMonostroma angicava Kjellm.J. Shandong coll. Oceanology. 13 (1): 57–65.
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相关作者
Yongnan LI
Yue WANG
Xuwen WU
Linlin ZHANG
Qian LI
Pengcheng GAO
Zhenjia WAN
Shan GAO
相关机构
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao)
University of Chinese Academy of Sciences
Department of Marine Organism Taxonomy & Phylogeny, Institute of Oceanology, Chinese Academy of Sciences
CAS and Shandong Province Key Laboratory of Experimental Marine Biology & Center of Deep Sea Research, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences