Isolation and cultivation of the vegetative cells ofPorphyra haitanensis

Wang Sujuan; Wang Guangyuan; Sun Yunlong; Lu Anming

doi:10.1007/BF02843815

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Isolation and cultivation of the vegetative cells ofPorphyra haitanensis

OriginalPaper | Updated：2023-05-16

- Isolation and cultivation of the vegetative cells ofPorphyra haitanensis
- Isolation and cultivation of the vegetative cells ofPorphyra haitanensis
- 海洋湖沼学报（英文） 1987年5卷第4期页码：333-339
- Affiliations：
  
  Shanghai Fisheries University
- Author bio：
- Funds：
- DOI：10.1007/BF02843815
  中图分类号：
- 纸质出版：1987
- Accepted：
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Isolation and cultivation of the vegetative cells ofPorphyra haitanensis[J]. 海洋湖沼学报（英文）, 1987,5(4):333-339.

Sujuan, W., Guangyuan, W., Yunlong, S. et al. Isolation and cultivation of the vegetative cells ofPorphyra haitanensis, ., Chin. J. Ocean. Limnol. 5, 333–339 (1987). https://doi.org/10.1007/BF02843815
Isolation and cultivation of the vegetative cells ofPorphyra haitanensis[J]. 海洋湖沼学报（英文）, 1987,5(4):333-339. DOI： 10.1007/BF02843815.

Sujuan, W., Guangyuan, W., Yunlong, S. et al. Isolation and cultivation of the vegetative cells ofPorphyra haitanensis, ., Chin. J. Ocean. Limnol. 5, 333–339 (1987). https://doi.org/10.1007/BF02843815 DOI：

摘要

Abstract

This research deals mainly with the use of the vegetative cells of a marine red algaPorphyra haitanensis (laver) as seeds and their culture into young thalli for further cultivation in the sea. The experimental process was as follows. Enzymatically isolated vegetative cells were attached to rope substrates and cultured in the laboratory for a month until they became about 0.2–0.5 cm long sporelings and were then attached (7–15 sporelings per cm of rope) to nets for removal to the open sea for cultivation. A month after culturing

the thalli reached a maximum length of 50 cm (average of 20–30 cm). The thalli grew faster as the water temperature dropped from 21°C to 17°C. It was proved that the vegetative cells isolated from a small thallus about 5 cm long could grow up into normal thalli after being kept frozen for a year. The results of this study show that vegetative cells can be used as new seeds to simplify the production of laver sporelings. This technique is a significant advance in the field ofPorphyra culture.

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references

Fujian Province Bureau of Fisheries, 1979. Artificial Culture ofPorphyra haitanensis. People’s Press of Fujian Province, pp. 28–33, 65–78. (in Chinese)

Fujita, Y. and Migita, S., 1985. Isolation and culture of protoplasts from some seaweeds.Bull. Fac. Fish. Nagasaki Univ. 57: 39–45.4

Institute of Oceanology, Academia Sinica, 1978. Artificial Culture ofPorphyra yezoensis. Science and Technology Press, Beijing, pp. 59–61, 88–93. (in Chinese)

M. Polne-Fuller, M. Biniaminov and A. Gibor, 1984. Vegetative propagation ofPorpnyra perforata.Hydrobiologia 116/117:308–313.

Saga, N., 1984. Isolation of protoplasts from edible seaweeds.Bot. Mag. Tokyo 97: 423–427.

Saga, N., and Sakai, Y., 1984. Isolation of protoplasts fromLaminaria andPorphyra.Bull. Jap. Soc. Sci. Fish. 50: 1085.

Tang Yanlin, 1982. Isolation and cultivation of the vegetative cells and protoplasts ofPorphyra suborbiculata Kjellm.Journal of Shandong College of Oceanology 6(4): 45–50. (in Chinese)

Wang Sujuan, Zhang Xiaoping, Xu Zhidong and Sun Yunlong, 1986. Study on the cultivation of cells and protoplasts ofPorphyra haitanensis.Oceanologia et Limnologia Sinica 17(3):217–221. (in Chinese with English abstract)

Wang Sujuan, Xu Zhidong, Wang Guangyuan and Xia Zhenao, 1986. Ultrastructural study on protoplasts ofPorphyra haitanensis.Marine Sciences 10(4): 21–24. (in Chinese with English abstract)

Zhao Huandeng and Zhang Xuecheng, 1984. On the cultivation of the isolated vegetative cells ofPorphyra yezoensis Ueda.Journal of Fisheries of China 8(3): 197–202. (in Chinese)

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