Heat-shock protein 70 expression in shrimpFenneropenaeus chinensis during thermal and immune-challenged stress

Guo Zhenyu; Jiao Chuanzhen; Xiang Jianhai

doi:10.1007/BF02843633

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Heat-shock protein 70 expression in shrimpFenneropenaeus chinensis during thermal and immune-challenged stress

OriginalPaper | Updated：2023-05-16

- Heat-shock protein 70 expression in shrimpFenneropenaeus chinensis during thermal and immune-challenged stress
- Heat-shock protein 70 expression in shrimpFenneropenaeus chinensis during thermal and immune-challenged stress
- 海洋湖沼学报（英文） 2004年22卷第4期页码：386-391
- Affiliations：
  
  Laboratory of Experimental Marine Biology Institute of Oceanology CAS
- Author bio：
- Funds：
  
  Supported by the National Key Fundamental Research Program “Study of the Major Diseases and Resistance Mechanism of Mariculture Organisms”, NSFC, No. 30140017, and the international cooperative program “Immunaqua” by the European Commission, No. ICA4-CT-2001-10023.
- DOI：10.1007/BF02843633
  中图分类号：
- 纸质出版：2004
- Accepted：
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Heat-shock protein 70 expression in shrimpFenneropenaeus chinensis during thermal and immune-challenged stress[J]. 海洋湖沼学报（英文）, 2004,22(4):386-391.

Zhenyu, G., Chuanzhen, J. & Jianhai, X. Heat-shock protein 70 expression in shrimpFenneropenaeus chinensis during thermal and immune-challenged stress., Chin. J. Ocean. Limnol. 22, 386–391 (2004). https://doi.org/10.1007/BF02843633
Heat-shock protein 70 expression in shrimpFenneropenaeus chinensis during thermal and immune-challenged stress[J]. 海洋湖沼学报（英文）, 2004,22(4):386-391. DOI： 10.1007/BF02843633.

Zhenyu, G., Chuanzhen, J. & Jianhai, X. Heat-shock protein 70 expression in shrimpFenneropenaeus chinensis during thermal and immune-challenged stress., Chin. J. Ocean. Limnol. 22, 386–391 (2004). https://doi.org/10.1007/BF02843633 DOI：

摘要

Abstract

Using western immunoblotting we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimpFenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp. HSP70 was prominently induced in hepatopancreas and gills

but not in muscle

eyestalk and hemolymph

when the shrimp were exposed to heat shock andVibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress

which was elevated after acute heat shock exposure of 10°C above ambient temperature.

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references

Ananthan, J., A. L. Goldberg and R. Voellmy, 1986. Abnormal proteins serve as eukaryotic stress signals and trigger activation of heat shock genes.Science 232: 522–524.

Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72: 248–254.

Bosch, T. C. G., S. M. Krylow, H. R. Bode et al., 1988. Thermotolerance and synthesis of heat shock proteins: these responses are present inHydra attenuata but absent inHydra oligetis Proc. Natl. Acad. Sci. USA.85: 7927–7931.

Bosch, T. C. G., K. Gellner and G. Praetzel, 1991. The stress response in freshwater polypHydra.In: B. Maresca and S. Lindquist eds, Heat Shock, Springer-Verlag, Berlin. p. 133–142.

Clegg, J. S., K. R. Uhlinger, S. A. Jackson et al., 1998. Induced thermotolerance and the heat shock protein-70 family in the Pacific oysterCrassostrea gigas.Molecular Marine Biology and Biotechnology 7(1): 21–30.

Craig, E. A., J. S. Weismann and A. L. Horwich, 1994. Heat shock proteins and molecular chaperones: mediators of protein conformation turnover in the cell.Cell 78: 365–372

Deane, E. E., S. P. Kelly, I. N. K. Chow et al., 2000. Effect of a prolactin pharmacological stimulant (sulpiride) and suppressant (bromocriptine) on heat shock protein 70 expression in silver sea bream,Sparus sarba.Fish Physiology and Biochemistry 22: 125–133.

Fagan, M. B. and I. L. Weissman, 1996. Sequence and characterization of two HSP70 genes in the colonial protochordate Botryllus schlosseri.Immunogenetics 44: 134–142.

Frydman, J., E. Nimmesgern, K. Ohtsuka et al., 1994. Folding of nascent polypeptide chains in a high molecular mass assembly with molecular chaperones.Nature 370: 111–117.

Gallucci, S. and P. Matzinger, 2001. Danger signals: SOS to the immune system.Curr. Opin. Immunol. 13: 114–119.

Hahn, G. M. and G. C. Li, 1990. Thermotolerance, thermoresistance and thermosensitization.In: R. I. Morimoto, A. Tissieres and C. Georgopoulos ed, Stress Proteins in Biology and Medicine, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. p. 79–100.

Hightower, L. E., 1980. Cultured animal cells exposed to amino acid analogues or puromyein rapidly synthesize several polypeptides.J. Cell Physiol. 102: 407–427.

Hightower, L. E., 1991. Heat shock, stress proteins, chaperones and proteotoxicity.Cell 66: 191–197.

Iwama, G. K., P. T. Thomas, R. B. Forsythe, et al., 1998. Heat shock protein expression in fish.Reviews in Fish Biology and Fisheries 8: 35–56.

Laemmli, U. K., 1970. Cleavage of Structural proteins during the assembly of the head of bacteriophage T4.Nature 227: 680–685.

Langer, T., C. Lu, H. Echols et al., 1992. Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding.Nature 356: 683–689.

Li, G. C., L. G. Li, Y. K. Liu et al., 1991. Thermal response in rat fibroblasts stably transfected with the human 70-kDa heat shock protein-encoding gene.Proc. Natl. Acad. Sci. USA 88: 1681–1685.

Lindquist, S. and E. A. Craig, 1988. The heat-shock proteins.Annu. Rev. Genet. 22: 631–677

Morimoto, R. I., C. Hunt, S. Y. Huang et al., 1986. Organization, nucleotide sequence and transcription of the chicken HSP70 gene.Journal of Biological Chemistry 261: 12692–12699.

Morimoto, R. I., 1993. Cells in stress: transcriptional activation of heat shock genes.Science 259: 1409–1410.

Murray, P. J. and R. A. Young, 1992. Stress and immunological recognition in host-pathogen interactions.J. Bacteriol. 174: 4193–4196.

Parsell, D. A. and S. Lindquist, 1993. The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins.Annu. Rev. Genet. 27: 437–496.

Roberts, J. K. and J. L. Key, 1991. Isolation and characterization of a soybean HSP70 gene.Plant Molecular Biology 16: 671–683.

Rodriguez, J., V. Boulo, E. Mialhe et al., 1995. Characterization of shrimp haemocytes and plasma components by monoclonal antibodies.J. Cell Sci. 108: 1043–1050.

Roux, A. F., V. T. Nguyen, J. A. Squire et al., 1994. A heat shock gene at 14q22: mapping and expression.Human Molecular Genetics 3: 1819–1822.

Schröder, H., T. Langer, F. U. Hartl et al., 1993. DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein.EMBO J. 12: 4137–4144.

Skowyra, D., C. Georgopoulos and M. Zylicz, 1990. TheE. coli dnaK gene product, the hsp70 homolog, can reactivate heat-inactivated RNA polymerase in an ATP hydrolysis-dependent manner.Cell 62: 939–944.

Solomon, J. M., J. M. Rossi, K. Golic et al., 1991. Changes in HSP70 alter thermotolerance and heat-shock regulation inDrosophila.New Biol. 3: 1106–1120.

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