Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)[J]. 海洋湖沼学报(英文), 2004,22(4):381-385.
Pengzhan, Y., Quanbin, Z., Hong, Z. et al. Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)., Chin. J. Ocean. Limnol. 22, 381–385 (2004). https://doi.org/10.1007/BF02843632
Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)[J]. 海洋湖沼学报(英文), 2004,22(4):381-385. DOI: 10.1007/BF02843632.
Pengzhan, Y., Quanbin, Z., Hong, Z. et al. Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)., Chin. J. Ocean. Limnol. 22, 381–385 (2004). https://doi.org/10.1007/BF02843632DOI:
Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)
摘要
Abstract
As molecular weight controls the biological activities of polysaccharides
screening the optimal molecular weight of polysaccharides is important in drug research and application. In this study
hydrogen peroxide was employed as oxidant
and temperature
reaction time
and concentration of polysaccharides and hydrogen peroxide were examined for their effects on the preparation of polysaccharides in different molecular weights fromUlva pertusa. Our experiment suggested that the optimal degradation concentrations for polysaccharides and hydrogen peroxide were 2.5% (w/v) and 8.0% (v/v)
respectively. The range of degradation measured by relative viscosity was mainly controlled by temperature and time. Results revealed that 35°C was the optimal temperature for obtaining low-degradation samples
and 50°C was the most favorable temperature to accelerate the reaction to yield highly-degraded samples. Four samples in different molecular weights A
B
C and D were finally prepared. The controllability was evaluated by the relative standard deviation (RSD) of relative viscosity
and the peak molecular weights and the polydispersity indexes (Mw/Mn) of molecular weights were measured by high performance gel permeation chromatography (HPGPC).
关键词
Keywords
references
Chen, H. and K. Wang, 1997. Function and structure of glycoconjugates. Shanghai Medical University Press, Shanghai, China. p. 324–341. (in Chinese)
Chevolot, L., A. Foucault, F. Chaubet et al., 1999. Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity.Carbohydr. Res. 319: 154–165.
Crepon, B., F. Maillet and M. D. Kazatchkine, 1987. Molecular weight dependency of the acquired anticomplementary and anticoagulant activities of specifically substituted dextrans.J. Biomaterials 8: 248–253.
Guan, M., W. Lin and Y. Ding, 2001. Marine drugs-studies on the Chinese pharmacology newly in the great demand at the 21 century.Chin. J. Mar. Drugs 20: 1–5.
Mauray, S., E. de Raucourt, J. Talbot et al., 1998. Mechanism of factor IXa inhibition by antithrombin in the presence of unfractionated and low molecular weight heparins and fucoidan.Biochim. et Biophys. Acta 1387: 184–194.
Millet, J., S. C. Jouault, S. Mauray et al., 1999. Antithrombotic and anticoagulant activities of a low molecular weight fucoidan by the subcutaneous route.Thromb. Haemost. 81: 391–395.
Nishino, T., Y. Aizu and T. Nagumo, 1991. The influence of sulfate content and molecular weight of a fucan sulfate from the brown seaweedEcklonia kurome on its antithrombin activity.Thromb. Res. 64: 723–731.
Shi, R. X., Z. H. Xu and Z. E. Li, 2000. Antitumor activity of degradedchondrus ocellatus polysaccharide.J. Oceanol. Limnol. Sin. 31: 653–656.
Zhang, W. Q., P. H. Chai, W. Xia et al., 2000. Preparation of chito-oligosaccharide by synergistic oxidation of sodium hydrochlorite/hydrogen peroxide.J. East China Univ. Sci. Technol. 26: 425–428.
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相关作者
Haibing DING
Fenghua JIANG
Wei CAO
Chaonan GUO
Chengjun SUN
Dong CUI
Yong ZHANG
Heyong HUANG
相关机构
First Institute of Oceanography, Ministry of Natural Resources
Marine Ecology and Environmental Science Laboratory, Laoshan Laboratory
Frontier Science Center for Deep Ocean Mulitsphere and Earth System; Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education; and College of Chemistry and Chemical Engineering, Ocean University of China
School of Environment, Nanjing Normal University
Jiangsu Open Laboratory of Large-scale Scientific Instruments, Nanjing Normal University