Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium

Mei Junxue; Shen Songdong; Jiang Ming; Fei Xiugeng

doi:10.1007/BF02843147

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Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium

OriginalPaper | Updated：2023-05-16

- Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium
- Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium
- 海洋湖沼学报（英文） 2003年21卷第2期页码：166-170
- Affiliations：
  
  1. Marine Biotechnology Department Shandong University Weihai Branch
  2. Biology Department Suzhou University West Campus
  3. Qingdao Ocean University
  4. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Contribution No. 4492 from the Institute of Oceanology, Chinese Academy of Sciences.;Project supported by National Key Fundamental Research Program G1999012009, Innovation Fund for Agricultue 02EF216600682 and the KLEMB, CAS.
- DOI：10.1007/BF02843147
  中图分类号：
- 纸质出版：2003
- Accepted：
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Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium[J]. 海洋湖沼学报（英文）, 2003,21(2):166-170.

Junxue, M., Songdong, S., Ming, J. et al. Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium., Chin. J. Ocean. Limnol. 21, 166–170 (2003). https://doi.org/10.1007/BF02843147
Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium[J]. 海洋湖沼学报（英文）, 2003,21(2):166-170. DOI： 10.1007/BF02843147.

Junxue, M., Songdong, S., Ming, J. et al. Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium., Chin. J. Ocean. Limnol. 21, 166–170 (2003). https://doi.org/10.1007/BF02843147 DOI：

摘要

Abstract

It had been demonstrated that individual cells or protoplasts isolated fromPorphyra thallus by enzyme could develop into normal leafy thalli in the same way as monospores

and that isolated cells develop in different way in liquid and on semi-solid media. The authors observed the ultrastructure of isolated vegetative cells cultured on semi-solid media and compared them with those of monospores and isolated cells cultured in liquid media. The results showed that subcellular structures were quite different among cells in different conditions. In their development

isolated cells on semi-solid media did not show the characteristic subcellular feature of monospore formation

such as production of fibrous vesicles. Callus-like cells formed on semi-solid media underwent a distinctive modification in cellular organization. They developed characteristic cell inclusions and a special 2-layer cell covering. Golgi bodies

starch grains

mitochondria. Vacuoles were not commonly found in them.

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references

Chen, L. C. M., 1989. Cell suspension culture fromPorphyra linearis (Rhodophyta), a multicellular marine red alga.J. Appl. Phycol. 1: 153–159.

Chen, L. C. M., 1997.Porphyra cell suspension cultures, novel biotechnological approach for the production of hoshi-nori. Nat. Hist. Res., Natural History Museum and Institute, Chiba, Japan. Special Issue No.C3: 111–121.

Gall, E. A., Chiang, Y. M., Kloareg, B., 1993. Isolation and regeneration of protoplasts fromPorphyra dentata-and, Porphyra crispata.Eur. J. Phycol. 28: 277–283.

Hawkes, M. W., 1980. Ultrastructure characteristics of monospore formation inPorphyra gardneri (Rhodophyta).J. Phycol. 16: 192–196.

Mei, J. X. and Fei, X. G., 2001. The ultrastructure of separated and cultured cell ofPorphyra yezoensis.Chin. J. Oceanol. Limnol. 19(1): 35–39.

Polne-Fuller, M., Biniamonov, M., Gibor, A., 1984. Vegetative propagation ofPorphyra perforata.Hydrobiologia. 116/117: 308–313.

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Wang, S. J., Yan, X. H., 1990. Observation on the monospore-like cells in the somatic cell culture ofPorphyra haitanensis.Oceanologia et Limnologia Sinica. 21(3): 166–169. (in Chinese with English Abstract)

Zhao, H., Zhang, X., 1981. Isolation and cultivation of the vegetative cells ofPorphyra yezoensis Ueda.J. Shandong College Oceanogr. 11(1): 61–66. (in Chinese)

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