Purification and characterization of lectin from humoral fluids ofCharybdis feriatus

Dai Congjie; Wang Guizhong; He Jianfeng; Li Shaojing; Huang Huiyang

doi:10.1007/BF02842855

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Purification and characterization of lectin from humoral fluids ofCharybdis feriatus

OriginalPaper | Updated：2023-05-16

- Purification and characterization of lectin from humoral fluids ofCharybdis feriatus
- Purification and characterization of lectin from humoral fluids ofCharybdis feriatus
- 海洋湖沼学报（英文） 2006年24卷第4期页码：390-394
- Affiliations：
  
  1. Department of Oceanography State key Lab of Marine Environmental Sciences Xiamen University
  2. Polar Research Institute of China
  3. Department of Biology Quanzhou Normal College
- Author bio：
- Funds：
  
  Supported by Project of Technology Bureau of Quanzhou of Fujian Province (Z200239), and by the Fujian Natural Science Foundation (B0410027)
- DOI：10.1007/BF02842855
  中图分类号：
- 网络首发：2013-01-31，
  
  纸质出版：2006
- Accepted：
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Purification and characterization of lectin from humoral fluids ofCharybdis feriatus[J]. 海洋湖沼学报（英文）, 2006,24(4):390-394.

Congjie, D., Guizhong, W., Jianfeng, H. et al. Purification and characterization of lectin from humoral fluids ofCharybdis feriatus, ., Chin. J. Ocean. Limnol. 24, 390–394 (2006). https://doi.org/10.1007/BF02842855
Purification and characterization of lectin from humoral fluids ofCharybdis feriatus[J]. 海洋湖沼学报（英文）, 2006,24(4):390-394. DOI： 10.1007/BF02842855.

Congjie, D., Guizhong, W., Jianfeng, H. et al. Purification and characterization of lectin from humoral fluids ofCharybdis feriatus, ., Chin. J. Ocean. Limnol. 24, 390–394 (2006). https://doi.org/10.1007/BF02842855 DOI：

摘要

Abstract

To search new sources of lectin

an experiment on lectin distribution in humoral serum of crabCharybdis feriatus (in short

CFL) was conducted March

2002. When adding solid ammonium sulfate into the fluids up to 50% saturation at 4°C

most CFL activity showed precipitates who were then continually extracted by ammonium sulfate of different concentrations. The supernatant

which was called primary CFL fluids

was given a 17.60-fold purification and 45.70% recovery of total activity. Finally

by using Sephadex G-100 column chromatography

the CFL in the primary CFL fluid was highly purified. Compared to the original humoral fluids

the last purified CFL got a 203.90-fold purification and 30.48% recovery of total activity

and demonstrated a single band on SDS-PAGE. In the same time

the purified CFL was detected for agglutination activity with 7 kinds of animal erythrocytes. Other characterization

such as sugar inhibition

and the effect of temperature on the agglutination activity of CFL were also studied. Our results indicate that agglutination activity of CFL was influenced by sugar and temperature.

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references

Arason, G. J., 1996. Lectins as defense molecules in vertebrates and invertebrates.Fish Shellf Immunol. 6: 277–289.

Blum, H., H. Beier and H. Grass, 1987. Improved silver staining of protein-plants. RNA and DNA in polyacrylamide gels.Electrophoresis. 8: 93–99.

Cooper, E. L., B. Rinkevich, G. Uhlenbruck and P. Valembois, 1992. Invertebrate immunity: another view-point, Scand.J. Immunol. 35: 247–266.

Department of Biology Sun Yat-sen University, 1978. Technology of Biochemistry-an introduction. People’s Education Press, Beijing.

Hall, J. L. and D. T. Rowlands, 1974. Heterogeneity of lobster agglutinins: I. Purification and physicochemical characterization.Biochemistry 13: 821–827.

Kawabata, S. and S. Iwanaga, 1999. Role of lectins in the innate immunity of horseshoe crab.Dev. Comp. Immunol. 23: 391–400.

Laemmli, U.K., 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4.Nature 227: 680–2.

Marques, M. R. F. and M. A. Barracco, 2000. Lectins, as non-self recognition factors, in crustaccans.Aquaculture 191: 23–44.

Matsushita, M., 1996. The lectin pathway of the complement system.Microbiol. Immunol. 40: 887–893.

Olafsen, J. A., 1996. Lectins: models of natural and induced molecules in invertebrates.Advances in Comparative and Environmental Physiology 24: 49–76.

Vasta, G. R., H. Ahmed, N. E. Fink, M. T. Elola, A. G. Marsh, A. Snowden and E. W. Odom, 1994. Animal lectins as self:non-self recognition molecules. Biochemical and genetic approaches to understanding their biological roles and evolution.Ann. N.Y. Acad. Sci. 15(712): 55–73.

Vasta, G. R., M. Quesenberry, H. Ahmed and N. O’Leary, 1999. C-type lectins and galectins mediate innate and adaptative immune functions: their roles in the complement activation pathway.Dev. Comp. Immunol. 23: 401–420.

Vazquez L., C. Sierra, S. Juarez, C. Agundis, A. Zavala and E. Zenteno, 1998. Mecanismos de immunidad encrustaceos.Inter Ciencia 23: 344–348.

Wilson, R., C. Chen and N. A. Ratcliffe, 1999. Innate immunity in insects: the role of multiple, endogenous humoral fluids lectins in the recognition of foreign invaders in the cockroach,Blaberus discoidalis.J. Immunol. 162: 1590–1596.

Yeaton, R. W., 1981. Invertebrate lectins: I: Occurrence.Dev. Comp. Immunol. 5: 391–402.

Zhao, G. D., X. Gao and B. X. Su, 1992. The preliminary studies of a lectin (MML) from sea clam (Meretrix meretrix) 1: The distribution in the sea clam tissues of MML and its purification.Acta Zhongshan Univ. (Nat. Sci.)31(1): 86–91.

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