Heterologous gene expression driven by carbonic anhydrase gene promoter inDunaliella salina

Chai Yurong; Lu Yumin; Wang Tianyun; Hou Weihong; Xue Lexun

doi:10.1007/BF02842849

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Heterologous gene expression driven by carbonic anhydrase gene promoter inDunaliella salina

OriginalPaper | Updated：2023-05-16

- Heterologous gene expression driven by carbonic anhydrase gene promoter inDunaliella salina
- Heterologous gene expression driven by carbonic anhydrase gene promoter inDunaliella salina
- 海洋湖沼学报（英文） 2006年24卷第4期页码：352-357
- Affiliations：
  
  Medical College Zhengzhou University
- Author bio：
- Funds：
  
  Supported by National High-Tech Research and Development Program of China (863 Program, No. 2002AA628050) and National Natural Science Foundation of China (No. 30270031).
- DOI：10.1007/BF02842849
  中图分类号：
- 网络首发：2013-01-31，
  
  纸质出版：2006
- Accepted：
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Heterologous gene expression driven by carbonic anhydrase gene promoter inDunaliella salina[J]. 海洋湖沼学报（英文）, 2006,24(4):352-357.

Yurong, C., Yumin, L., Tianyun, W. et al. Heterologous gene expression driven by carbonic anhydrase gene promoter inDunaliella salina, ., Chin. J. Ocean. Limnol. 24, 352–357 (2006). https://doi.org/10.1007/BF02842849
Heterologous gene expression driven by carbonic anhydrase gene promoter inDunaliella salina[J]. 海洋湖沼学报（英文）, 2006,24(4):352-357. DOI： 10.1007/BF02842849.

Yurong, C., Yumin, L., Tianyun, W. et al. Heterologous gene expression driven by carbonic anhydrase gene promoter inDunaliella salina, ., Chin. J. Ocean. Limnol. 24, 352–357 (2006). https://doi.org/10.1007/BF02842849 DOI：

摘要

Abstract

Dunaliella salina

a halotolerant unicellular green alga without a rigid cell wall

can live in salinities ranging from 0.05 to 5 mol/L NaCl. These features ofD. salina make it an ideal host for the production of antibodies

oral vaccine

and commercially valuable polypeptides. To produce high level of heterologous proteins fromD. salina

highly efficient promoters are required to drive expression of target genes under controlled condition. In the present study

we cloned a 5′ franking region of 1.4 kb from the carbonic anhydrase (CAH) gene ofD. salina by genomic walking and PCR. The fragment was ligated to the pMD18-T vector and characterized. Sequence analysis indicated that this region contained conserved motifs

including a TATA- like box and CAAT-box. Tandem (GT)n repeats that had a potential role of transcriptional control

were also found in this region. The transcription start site (TSS) of theCAH gene was determined by 5′ RACE and nested PCR method. Transformation assays showed that the 1.4 kb fragment was able to drive expression of the selectable bar (bialaphos resistance) gene when the fusion was transformed intoD. salina by biolistics. Northern blotting hybridizations showed that the bar transcript was most abundant in cells grown in 2 mol/L NaCl

and less abundant in 0.5 mol/L NaCl

indicating that expression of the bar gene was induced at high salinity. These results suggest the potential use of theCAH gene promoter to induce the expression of heterologous genes inD. salina under varied salt condition.

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references

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