In vitro study on cytotoxic effects of the organopho-sphorous pesticide profenofos on the gill cell line, FG-9307, of the flounder (Paralichihys olivaceus)

Li Hong-yan; Zhang Shi-cui; Jiang Ming; Wang Min

doi:10.1007/BF02842790

Your Location：

Home >

Browse articles >

In vitro study on cytotoxic effects of the organopho-sphorous pesticide profenofos on the gill cell line, FG-9307, of the flounder (Paralichihys olivaceus)

OriginalPaper | Updated：2023-05-16

- In vitro study on cytotoxic effects of the organopho-sphorous pesticide profenofos on the gill cell line, FG-9307, of the flounder (Paralichihys olivaceus)
- In vitro study on cytotoxic effects of the organopho-sphorous pesticide profenofos on the gill cell line, FG-9307, of the flounder (Paralichihys olivaceus)
- 海洋湖沼学报（英文） 2001年19卷第1期页码：57-62
- Affiliations：
  
  Department of Marine Biology Ocean University of Qingdao
- Author bio：
- Funds：
  
  This work was partially supported by the Marine 863 Project (No. 819-04-06).
- DOI：10.1007/BF02842790
  中图分类号：
- 纸质出版：2001
- Accepted：
Scan QR Code
In vitro study on cytotoxic effects of the organopho-sphorous pesticide profenofos on the gill cell line, FG-9307, of the flounder (Paralichihys olivaceus)[J]. 海洋湖沼学报（英文）, 2001,19(1):57-62.

Hong-yan, L., Shi-cui, Z., Ming, J. et al. , In vitro study on cytotoxic effects of the organopho-sphorous pesticide profenofos on the gill cell line, FG-9307, of the flounder (Paralichihys olivaceus)., Chin. J. Ocean. Limnol. 19, 57–62 (2001). https://doi.org/10.1007/BF02842790
In vitro study on cytotoxic effects of the organopho-sphorous pesticide profenofos on the gill cell line, FG-9307, of the flounder (Paralichihys olivaceus)[J]. 海洋湖沼学报（英文）, 2001,19(1):57-62. DOI： 10.1007/BF02842790.

Hong-yan, L., Shi-cui, Z., Ming, J. et al. , In vitro study on cytotoxic effects of the organopho-sphorous pesticide profenofos on the gill cell line, FG-9307, of the flounder (Paralichihys olivaceus)., Chin. J. Ocean. Limnol. 19, 57–62 (2001). https://doi.org/10.1007/BF02842790 DOI：

摘要

Abstract

The FG-9007 cell line derived from the gill of flounderParalichthys olivaceus was used in the present study to determine the acute cytotoxic effects of the organophosphorous pesticide

profenofos. It was found that the cell growth rate was markedly reduced by profenofos at the concentrations of 2.5

5 and 7.5 mg/L tested; and that the fine structures of the cells were also altered by profenofos

as evidenced by dilation of nuclear membranes and mitochondria cristae

and presence of enlarged lysosomes with engulfed organelles and numerous vacuoles in the cytoplasm. Probably

mitochondria

the cell energy-generating sites

are the most prominent sites of profenofos cytotoxity in the cells. This seems to be the first report of the use of marine fish cell line for evaluation of the acute in vitro cytotoxicity of organophosphorus pesticide.

关键词

Keywords

references

Anderson, S. W., Sadinski, L., Shugart P. et. al., 1994. Genetic and molecular ecotoxicology: A research framework.Environ. Health Perspect. 102: 3–8.

Babich, H., Borenfreund, E., 1987a. In vitro cytotoxicity of organic pollutants to bluegill sunfish (BF-2) cells.Environ. Res. 42: 229–237.

Babich, H., Borenfreund, E., 1987b. Structure-activity relationship (SAR) models establishedin vitro with the neutral red cytotoxicity assay.Toxicol. in vitro 1: 3–9.

Babich, H., Puener, J. A., Borenfreund, E., 1986. In vitro cytotoxicity of metals to bluegill (BF-2) cells.Arch Environ Contam Toxicol 15: 31–37.

Babich, H., Rosenberg, D. W., Borenfreund, E., 1991. In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida).Ecotoxicol. Environ. Sof. 21: 327–336.

Bols, N. C., Boliska, S. A., Dixon, D. G. et al., 1985. The use of fish cell cultures as an indication of contaminant toxicity to fish.Aquat. Toxicol. 6: 147–155.

Chen, H. H., Hsueh, J. L., Huang, C. C., 1981. Induction of sister-chromatid exchanges and cell cycle delay in cultured mammalian cells treated with eight organophosphorous pesticides.Mutat. Res. 88: 307–316.

el Nahas, S. M., de Hondt, H. A., Abdou, H. E., 1989. Chromosome aberrations in spermatogonia and spenn abnormalities in Curacron-treated mice.Mutat Res. 222: 409–414.

el-Sebae, A. H., Salem, M. H., el-Assar, M. R. et al., 1988. In vitro effect of profenofos, fenvalerate and dimilin on protein and RNA biosynthesis by rabbit liver and muscle tissues.J. Environ. Sci. Health 23: 439–451.

Fahmy, M. A., Abdalla, E. F., 1998. Genotoxicity evaluation of buprofezin, petroleum oil and profenofos in somatic and germ cells of male mice.J. Appl. Toxicol. 18: 301–305.

Gabrielides, G. P., 1995. Pollution and the Mediterranean Sea.Wat. Sci. Tech. 32: 1–10.

Gomes, J., Dawodu, A. H., Lloyd, O., 1999. Hepatic injury and disturbed amino acid metabolism in mice following prolonged exposure to organophosphorous pesticides.Hum. Exp. Toxicol. 18: 33–37.

Ishaaya, I., 1993. Insect detoxifying enzymes: their importance in pesticide synergism and resistance.Arch Insect Biochem. Physiol. 22: 263–276.

Kocan, R. M., Landolt, M. L., Sabo, K. M., 1979. In vitro toxicity of eight mutagens/carcinogens for three fish cell lines.Bull Environ. Contam. Toxicol. 23: 269–274.

Marion, M., Denizeau, F., 1983. Rainbow trout and human cells in culture for the evaluation of the toxicity of aquatic pollutants. A study with lead.Aquat Toxicol 3: 47–60.

Rachlin, J. W., Perlmutter, A., 1968. Fish cells in culture for study of aquatic pollutants.Water Res. 2: 409–414.

Tong, S. L., Li, H., Miao, H. Z., 1997. The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounderParalichthys olivaceus.Aquaculture 156: 327–333.

UNEP (United Nations Environment Programme), 1991. Assessment of the state of pollution of the Mediterranean Sea by organophosphorous compounds. MAP Technical Reports Series No. 58

Viarengo, A., 1985. Biochemical effects of trace metals.Mar. Pollut. Bull. NS 16: 153–158.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Protective effects of Ecklonia cava extract on the toxicity and oxidative stress induced by hair dye in in-vitro and invivo models

Comparative study of the hemolytic and cytotoxic activities of nematocyst venoms from the jellyfish Cyanea nozakii Kishinouye and Nemopilema nomurai Kishinouye

Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF

In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus

Chemical profile of the secondary metabolites produced by a deep-sea sediment-derived fungus Penicillium commune SD-118